Questions tagged [vector]

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5
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1answer
70 views

Are there any documented instances of coronaviruses being directly transmitted from bats to humans?

Many human coronaviruses have ancestral host origins in species of bat. However, all instances I am aware of identified other animals as intermediary vectors: SARS-CoV: Human ← Palm Civet / Raccoon ← ...
4
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0answers
88 views

Boost of Janssen COVID-19 Vaccine increasing Immune Response to Vector

I have been looking for published, or even pre-print, data that evaluates the serological response to a boost of the Janssen COVID-19 Vaccine against the adenoviral vector Ad26 rather than the ...
3
votes
1answer
112 views

Debug Fresno; why are the released mosquitos said to be sterile?

I saw in the news that Verily Life Sciences will participate in an experimental release of sterile male aedes aegypti mosquitos in Fresno, California. Aedes aegypti is considered an invasive species ...
2
votes
1answer
87 views

Where can I find disease diagnosis datasets?

For an epidemiological study, I'm looking for datasets for any kind of vector-borne disease (i.e. West Nile Virus, Malaria, etc.), or any parasites that are dependent on intermediate hosts (i.e. ...
2
votes
1answer
16 views

Comparing angles between population vectors of neural activity

I am reading this paper on fear conditioning, where the following is given: The n-dimensional population vector (activity of n neurons) evoked by the conditioned stimulus (CS+, auditory tone) before ...
0
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1answer
135 views

Coronavirus origin could be bat, why this happen while people have been eating bats for centuries? [closed]

Sorry for my lack of understanding, Why now, when people been eating bats for centuries? If bats are simply a vector, where does that virus come from?
0
votes
1answer
403 views

Alkaline Phosphatase and Ligase Protocol for Cloning

In the image the circular molecule is a restricted vector and the linear red molecule is a DNA insert. I found this protocol in my lessons notes, but I don't understand how it is possible that ligase ...
0
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0answers
34 views

Could the EDTA coating of tubes kill blood fed mosquitoes?

Problem is as follows: culex pipiens fed with freeze-thawed cattle blood sampled in 9ml EDTA tubes enjoy their meal and thrive. Same population fed with freeze-thawed human blood sampled in 4 ml EDTA ...
0
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0answers
9 views

In-plane rotation of vectors of neural population responses

In this paper on fear conditioning, the following is given: The n-dimensional population vector (activity of n neurons) evoked by the conditioned stimulus (CS+, auditory tone) before conditioning The ...
0
votes
0answers
35 views

Is there any commercial vector technology platform for boosting recombinant protein production?

I am a novice in biomedical industry. Our team would like to search new vector technology for boosting recombinant protein production. It's difficult to google a firm that provide those plasmids. Dose ...
0
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0answers
60 views

Cloning using pET28a and Protein Expression in DH5alpha and BL21

Can someone please direct me to an e-resource or a book that will help a newbie like me learn in depth about Cloning using pET28a and Protein Expression in DH5alpha and BL21. Though I have done ...
0
votes
1answer
49 views

How do I reverse my insert within a plasmid?

I have a plasmid with: ...T7pro. -- RBS -- XBa1 cut site -- ProDpro. -- RBS -- AmilCP -- T -- Spe1 cut site -- T... How do I design primers to reverse the portion in bold, such that the T7 and ProD ...
-1
votes
1answer
46 views

Unusual terminology of vector used

What is the significance of a+ in the name pET-28 a(+)? Is there any a- strain and differences between a,a+,a-?
-1
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1answer
52 views

What are the advantages using SUMO vector for expression? [closed]

My question is : What are the advantages using SUMO vector for expression?