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Questions tagged [virology]

Virology deals with the study of viruses, infectious entities that require the machinery of a host cell to replicate.

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What percentage of cells in the human body are infected during a non-fatal viral infection?

I understand that this would be a very rough estimate, but approximately how many cells in the human body are infected during an average non-fatal viral infection before the infection is eliminated by ...
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Virucides - Herbal and otherwise, for HHV6 type virus or any inner-cell virus

Does anyone know where I can find a reliable list of virucides (not anti-virus) that can be used by humans? This virucide must be able to affect viruses that are already inside the cells. St John's ...
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Do we know that PrEP actually prevents infection, rather than masking it?

Pre-exposure prophylactic treatment (PrEP) for HIV is commonly said to reduce infection rates by more than 75%. Similar treatments for already-infected patient treatments do not cure HIV, but reduce ...
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Can HBV pseudotyped oncolytic viruses be used to treat hepatocellular carcinoma?

Chronic hepatitis B is the leading cause of hepatocellular carcinoma (HCC). Oncolytic virotherapy (OV) is an emerging tool to treat cancer. However, one challenge of OV is that our immune system may ...
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How does taking drugs speed up the development of drug resistant or viruses?

First, here is what I know: Some infectious illnesses, such as HIV or Hepatitis B, can be treated with the help of some drugs. The problem is that the virus sometimes undergoes mutations which grant ...
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Do symptoms of common cold infections correlate with the infectious agent?

The common cold - in summary, an acute upper respiratory tract viral infection - is one of the most frequent viral infections in humans and can be caused by about 200 viral types [Eccles (2005)]. ...
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Should gene therapy safety protocol include isolation?

In the case of a gene therapy trial where viral vectors are used to deliver genes into mammalian cells, including humans, should biosafety and ethical protocols include isolation of the patient as a ...
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Enzyme inhibitors against common cold viruses

Would some inhibitors of viral enzymes work against common cold viruses? Are there any studies? What could a treatment look like?
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Are there examples of non-pathogenic RNA viruses?

There do seem to be some non-pathogenic DNA viruses, for example Foamy virus. Non-pathogenic viruses would be great to use for gene therapy applications, except DNA viruses incorporate themselves into ...
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Mosquito vs Human: Swapping the roles

Is there a known mosquito-specific lethal (for some relevant species of mosquitos) virus that you could safely (for the humans) put into the blood of living humans? Is it technically possible to ...
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Can viruses replicate in a cell that's already dead?

Suppose a virus randomly lands on a cell that's just lying around. This cell may have just recently died, or died a while ago. This cell may also be part of tissue of a dead organism.* Does the virus ...
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Can 3% hydrogen peroxide be used as effective disinfectant and antiseptic?

I was curious to see whether 3% hydrogen peroxide can be used as antimicrobial and virucidal product for daily use in home. There are several disinfecting products in the market claim that use of ...
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Do all +ssRNA viruses have similar structures and life cycles?

Take HCV for example. HCV doesn't package any enzymes in it's virions. When it infects a cell, it first translates its RNA genome. It's RdRp is synthesized with other non-structural proteins. Then its ...
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HIV and open reading frames

In Wilk et al. 2001 I saw that HIV has 3 open reading frames. In the Watts et al. 2009, I noticed they mentioned HIV has 9 open reading frames. I don't understand this very well. e.g. ...
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Virus plaque assay, pfu/ml calculation on plates with too little plaques formed

I am a student, so I hope it is okay to put this question here. I've done the virus plaque assay in a practical recently and have a few questions regarding the plaque count for pfu calculation. In ...
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Why do some viruses have only one serotype, but others have many?

Some viruses, like the virus that causes mumps, have only a single serotype, meaning that variants of the disease are immunologically identical. However, other viruses, like the influenza virus family,...
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largest and smallest gram positive, gram negative and fungal pathogens

As I stumbled through this question: A 16-year-old female patient presented to her family physician with a complaint of an abnormal vaginal discharge and pruritus (itching). The patient ...
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What is the difference between operator mutant and repressor mutant lambda viruses?

I wanted to know what the difference between the operator mutant and repressor mutant lambda bacteriophages is and how do they act on E. coli cells.
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How to estimate MOI for a complex cell type

I am currently confused about the estimation of multiplicity of infection (MOI), here is an example in a scientific article in figure 3b, they tested the virus, and get estimated MOI based on this: ...
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Are exosomes useful as a transfection or delivery mechanism in gene editing?

The use of viruses as transfection or delivery agents for gene editing (CRISPR/cas9, etc) is well known. However, one problem with using viruses to deliver DNA into cells is the possibility of ...
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Cat herpes and behavior

We have 2 cats, aptly named "Fatty" and "Tiny". "Fatty" is a 6 year old house cat that hasn't seen the outside world in years. "Tiny" is a cat we rescued about 45 days ago and she's roughly 2.5 ...
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What chemicals will kill P1 Phage but not E. coli?

I'm working with E. coli and P1 Phage. I'm wondering if there is some chemical agent that will kill or disable P1 but leave E. coli untouched? It is not enough to just prevent infection. It must ...
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Concerning Rabies can an animal feeding on rabid bat transmit right away?

Playful kitten found biting and toying with a dead bat. Lets say the kitten nips and plays rough with a human right after biting and toying with the bat. Will a potential rabies virus transmit from ...
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A Designer Virus

An article at Wikipedia describes color changes that viruses have produced in tulips (without any human intention). And another article (elsewhere on the web) describes viruses that were designed to ...
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Which viruses are fatal to E.Coli?

I am currently doing a bit of research into which viruses are specifically fatal to the E.Coli bacterium. Apart from the fact that this is merely interesting information for me, I would like to know ...
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How does sGP of Ebola virus help it to evade host humoral immunity?

During Ebola infection, the viruses secret a lot of sGP. What's its function? Since anti GP antibodies are effective at inhibiting Ebola infection, wouldn't sGP stimulate host immune system to produce ...
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Bacteriophage lysis in mammalian cells

I have read many articles on bacteriophages (like the lambda phage1) being used for transferring genes into mammalian cells, but none of them mention any sort of lysis of the cells even though in ...
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Can HPV be infectious after it's been exposed to environment?

From what I know, HPV virus cannot be cultured I don't think it's possible to say if a sample has the potential to infect or not but what does it mean when they say HPV virus particles could persist ...
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Barcode exchange between neighbouring cells

I am currently interested in equipping animal cells in a tissue with individual barcodes. These barcodes should get amplified in the cells (not to a cell degrading extent, of course), and also ...
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How reliable is the life cycle information on PhagesDB?

If I'm understanding this correctly, PhagesDB users choose the Cluster/Subcluster during the upload, and the life cycle type is assigned solely based on this data. How reliable is that information? ...
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Do viruses go viral?

Wikipedia defines a viral phenomenon as: ... objects or patterns that are able to replicate themselves or convert other objects into copies of themselves when these objects are exposed to them. ...
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Mechanism Behind Viral Capsid Breakdown When In Cell

In the example of a retrovirus, after the lipid bilayer fuses with the membrane of the cell, the capsid dissolves releasing the viral content. What is the mechanism behind the capsid dissolving? I ...
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Is a lower wavelength UV-C more effective than higher wavelength for sanitization?

Is it more beneficial to use a lower wavelength UV-C bulb? For example, using a 180 nm UV-C bulb instead of 254 nm. I know the 180 nm bulb will produce ozone, which is very useful for filtration of ...
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What is the difference between a virus and virion

What is the difference between a virus and virion. Correct me if i am wrong but i think that one can simply say that " virus outside a cell is called a virion" and that this stage is used to go from ...
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How long does HIV take to penetrate the mucosae?

In articles, such as this, the biology of the HIV infection is explained. However, i haven't been able to find any research explaining how long the virus takes to penetrate the cell layer. The article ...
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Eveloped virus identification

Is Hemadsorption Innhibition enough to identify all enveloped virus? Or are there cases in which Hemadsorption innhibition will not work?
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How to dissociate a viral particle into constituent proteins?

Is it possible to take purified a virus particle and dissociate it into a mixture of its constituent proteins (and nucleic acids)? I am wondering if it would be possible to disassemble the particle ...
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Do influenza viruses obey a Hayflick limit similar to human cells .. or do they spread and divide forever like HeLa?

I would think the latter since they are so simple and are parasitic but even proteins break down over time .. only found a few answers on the net and it was over a decade ago but nothing in any papers ...
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Viral genetic potential

A question goes: The DNA of the adenovirus carries genes. Suggest what these genes code for. It's clear that they code for several specific protein components in the virus like the capsomeres, ...
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What feature of the norovirus makes it self-limiting?

Recently caught a stomach bug and began reading up on the internet. Every government health information service on the internet describes the norovirus as "self-limiting". Is this the biological ...
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What factors contributed to develop so many viral diseases in Europe?

Europe was epicenter of several viral diseases, such as Plague of Athens, influenza. These viruses went to other countries and infected may people, for example smallpox in Mexico. What factors ...
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Can we detect rabies during its incubation period?

Although some diseases are almost fatal and have a near 100% mortality, their incubation period can be very long. For example, rabies' incubation period can be days to years. I wonder when the ...
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What is the Biological Mechanism by Which a Red Heifer's Skin is Colored Red?

Obviously from this picture Picture of a Red Angus Heifer it is possible for there to be a red cow. So what is the biological mechanism that makes such a cow red? And why are regular cows only ...
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Bacteriophage isolation

I have environmental water sample from which i want to isolate bacteriophages. I've try to centrifugate several consecutive times. I've try with different speed (13000g and 15000g) and different ...
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What makes cardiac cells so sturdy?

We know that cardiac cells are extremely sturdy compared to pretty much any other tissue in the human body, not only because the heart as an organ beats for the entirety of the human lifespan, but ...
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How many antigens are there in dengue virus?

I need your help to know how many antigens are there in dengue virus? If you have any good reference about the molecular biology of dengue virus, Please write it down. Thank you.
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How effective will the synthesized virus be without epigenetic marks?

Will a de-novo synthesize infectious virus be as effective as the natural one? How important are epigenetic marks for the virus and is there a mechanism to restore them?
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Are there well studied examples of ERAD-mediated membrane insertion, especially from viruses?

Membrane insertion of transmembrane proteins typically requires highly hydrophobic alpha helixes at the N-terminus, N-terminal signal peptides, tail anchors, or a combination of the three. Byun, H., ...
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How do reoviruses manage to get nuceic acids inside their core given they stay in the host cell to replicate ?

To avoid host cell shut off and apoptosis, reoviruses produce capped mRNA internally within a core particle and the only viral genome released in the cytoplasm of the infected cells is ssRNA. Where ...
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How do antivirals function by altering the cells genetic material?

In my syllabus, it says that one mechanism of antiviral actions is that "Antiviral drugs may work by altering the cell’s genetic material so that the virus cannot use it to multiply". However, when I ...