All Questions
Tagged with western-blot protein-expression
4 questions
4
votes
0
answers
334
views
Relative densitometry from SDS PAGE
I'd like to perform densitometry on a Coomassie stained SDS PAGE gel to compare a recombinant protein's expression levels under two conditions. I'm using BioRad's Image Lab software.
My questions are ...
- 103
2
votes
0
answers
236
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Why HIS tag is not detected in WB or dot blot?
I would like to know why the recombinant protein with his tag at c terminus is not being detected neither in western blot or dot blot.
I'm trying to validate protein expression of four proteins. ...
- 971
3
votes
1
answer
952
views
Western blot alternatives
Given an in vivo sample from an experimental infection, I would like to see if a bacterial protein is present.
I've been thinking about Western Blot using sera from animals infected with the bacteria....
- 971
2
votes
1
answer
244
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can a bacterial protease inhibitor cocktail be used for Western Blotting involving HUVEC cells and HT-29 adenocarcinoma cells?
Dear fellow biochemists,
I need some advice on Western Blotting, more specifically the use of certain protease inhibitors with the RIPA cell lysis buffer and protease inhibitor cocktail. A Millipore ...
