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Questions tagged [yeast]

Yeasts are a polyphyletic group of unicellular fungi. Usually the term yeast is used synonymously with baker's yeast - Saccharomyces cerevisiae.

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Yeast integrating vectors: where to cut?

I understand that yeast integrating plasmids (YIps) must be linearized in order to promote homology-directed recombination. However I don't quite understand where. In the end, the external regions of ...
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Is it normal to see yeast cells stacked in the Z axis when counting yeast?

I just got started with yeast cell counting and was surprised to observe yeast cells that are at different z axis depths at 400x. That is, by adjusting the focus (not the x/y stage) I can make some ...
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Infecting yeast with viruses

Is there any virus, either natural or engineered, known to infect yeast through mechanisms similar to phages/human viruses, that is, by horizontal contamination mediated by extracellular viral ...
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How is Saccharomyces cerevisiae grown on acetate and ammonium able to produce TCA intermediates?

I am working with a metabolic model of Saccharomyces cerevisiae and I am studying its growth on acetate and ammonia. I am performing Flux Balance Analysis to compute the growth rate and then I am ...
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How can I estimate the total number of yeast cell in a medium?

So I want to run an experiment with yeast. I want to monitor the population as close as possibly in regards to its size. I am aware I could use a colorimeter or such, but I can only get a microscope. ...
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84 views

Continuous (yeast) culture system satisfying multiple criteria

There are multiple ways yeast cells can be grown in liquid cultures. Batch cultures are easy to prepare but cell density, medium composition and physiology change over time Chemostats allow to ...
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88 views

How can I measure the population growth of yeast?

So I will soon have to carry out an experiment. I thought about estimating the rate of growth of a populaiton of yeast, although I do not know if it is possible to at least estimate the number of ...
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Primer design for PRP24-TAPtag

I have a problem with my proteomics homework. I was asked to design primers used to create a homologous recombination box to create PRP24-TAPtag yeast. I know that homologous regions should be 40-50nt ...
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100 views

Can a plasmid cause cancer? [closed]

Can a plasmid cause cancer? According to wikipedia, plasmids are normally present in bacteria They (plasmids) are normally present in bacteria , and sometimes in eukaryotic organisms such as yeast [...
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260 views

The effect of sugar on rate of yeast Respiration

In my experiment, I am investigating the effect of different types of sugar on the rate of respiration of immobilized yeast (yeast trapped in calcium alginate balls). I am looking at glucose, fructose,...
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1answer
24 views

Where do I get an annotation file for yeast genome with ENSEMBL ids?

Even the annotation files hosted on the Ensembl site have systematic names (starting with Y..) instead of ENSEMBL ids (starting with EN..). I bet there would be a place where I can get an annotation ...
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1answer
65 views

How to measure the length of mitochondria from z stack fluorescent microscopy image?

I have been working on yeast cells to analyse the effect of DNA damaging agents on mitochondrial structure. I have imaged my culture treated with MMS for a period of 6 hours and while observing the ...
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Yeast transformation: is it necessary to remove template plasmid from PCR?

I'm frequently transforming budding yeast with PCR fragments. Normally, after PCR I digest with DpnI to remove the template plasmid. DpnI is a restriction enzyme which cuts methylated DNA, i.e. the ...
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97 views

rice wine yeast , rice wine mold culture expanding or propagating

i am very curious and wanted to know more about these yeast or mold that used to make chinese rice wine. any microbiologist can share their knowledge ? my friend from china brought this rice yeast ...
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1answer
165 views

Where do kombucha SCOBYs get nitrogen?

Kombucha can be brewed even with tiny amounts of inocculate, such as the little bit of sediment at the bottom of store-bought kombuchas. Over 1-2 weeks, this can grow into an inch thick film covering ...
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180 views

What is the practical difference between SC, SD and CSM?

Is there some logic to how SC, SD and CSM yeast dropout media differ from each other? I have encountered these three formulations in Sunrise Science Products' catalog, and it appears that the ...
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615 views

Can yeast survive without mitochondria?

Some yeast cells have very low mitochondria copy number (see number of mitochondria in non budding cell, you can count mtDNA). This makes them susceptible to fluctuations, and one can conceive that ...
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45 views

Yeast contamination with fungus patch

I am working on yeast mutant and I am observing their sporulation efficiency on agar plates. One of my plates got some dark green small fungus patches. I can't grow yeast again and dump this one with ...
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Why do some yeast flocculate on top an other at bottom?

When fermenting beer normally after the fermentation has completed the yeast flocculate and drop to the bottom. However occationally they form a foamy layer on top of the beer. The difference is ...
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Protein expression in K. lactis system

I am trying to express a protein in K. lactis Protein Expression Kit (E1000S - NEB) in the way that the protein is secreted into the medium. I know that I can get some protein but it is not active. As ...
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Yeast transformation plate confluent with growth

I have problem with yeast transformation. I tried to transform pyes3-tryp(my insert inside) into INVsc1 yeast cell by following the protocol in the manual for few times but failed(confluent with ...
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1answer
179 views

Is the CMV promoter active in yeast?

I wish to transfect yeast and want to control my efficiency. So I thought to use pEGFP-N1 as control. The EGFP is driven by a CMV promoter. Can yeast read CMV promoters. Thanks Hermann
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323 views

Is hydrochloric acid suitable for pickling food?

I was wondering whether it is possible to pickle food using hydrochloric acid (HCl), rather than acetic acid. I am not very familiar with the biology of the fermentation process involved in pickling ...
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1answer
128 views

How do I improve expression using the GAL1 promoter in S. cerevisiae above the “leaky” level I'm currently seeing?

I am attempting to express GFP in S. cerevisiae using the GAL1 promoter. I always grow an uninduced (in dextrose based SD medium) culture alongside my induced (in galactose based SD medium) culture. ...
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1answer
2k views

Why does yeast react the way it does to water combined with sugar?

I almost wanted to ask this in Cooking SE, but thought it would be better fit here. So I understand than when you mix yeast with sugar and warm water, you get CO2. When using baking powder, it's a ...
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1answer
34 views

Pichia pastoris and ornithine

Can Pichia pastoris (species of yeast used for recombinant protein) produce the non-protein amino acid Ornithine? Or does it express ornithine at all?
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53 views

What is the rate of mRNA decay in saccharomyces cerevisiae?

I am just looking for a number whether that is a mean or an estimate. I am having trouble finding this value on papers because the papers mention only the methods for calculating such a value. Is ...
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1answer
270 views

Yeast Strain with squalene synthase / farnesyltransferase (ERG9) gene deleted

I was interested in obtaining a yeast strain without the enzyme farnesyltransferase (Which catalyses conversion of FPP to Squalene). The Euroscraf database has thousands of yeast strains with ...
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Y285 Yeast Strain: Where to obtain from?

Often I see yeast strains mentioned like Y285 or Y301 etc. in papers. Is this some sort of universal notation? (Link to poster: https://ddd.uab.cat/pub/tfg/2014/119349/TFG_narcisferrerledo.pdf) How ...
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1answer
89 views

Enzyme Inhibitors: Using in vivo in yeast

I find listed on the Sigma-Aldrich site a large list of enzymes & corresponding inhibitors. e.g. For the enzyme squalene synthase the inhibitor listed is "Zaragozic acid A trisodium salt" Would ...
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1answer
107 views

Using PGAL10 promoter to overexpress specific yeast genes: How to?

I was reading a poster about expression boosting for Artemesinin production in yeast and it had this line: PGAL1 and PGAL10 have been used for the overexpression of all genes from the ...
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2answers
477 views

Earliest to use media after autoclaving?

I am autoclaving media to use later to inoculate yeast. How much time do I have to wait before using the media and adding the culture? Can I use it a few hours after autoclaving?
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1answer
5k views

What concentration of sugar is optimal for baker's yeast fermentation?

I'd like to do an experiment on what concentration of sugar would be best for fastest yeast fermentation. I'm thinking to put some active dry yeast into different concentrations of sugar water and ...
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662 views

The effects of auto-brewery syndrome

Why are some people affected so differently by auto-brewery syndrome differently if the syndrome seems to be caused by the single organism saccharomyces cerevisiae? It is known that the syndrome has ...
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2answers
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identifying which SNPs sit in TFBS (Yeast)

i have a set of ~11k SNPs for Saccharomyces cerevisiae, baker's yeast and i would like to identify which ones of these sit in transcription factor binding sites and if they do information on the ...
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1answer
7k views

Relationship between turgor pressure and osmotic pressure?

I would like to know if there is a relationship between osmotic pressure (inside and outside of a cell) and turgor pressure. If so, is there a way to formalize it mathematically? Thank you in advance,...
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2answers
90 views

yeast protein secondary structure

i have a fasta file of yeast orfs (from the SGD database) and i need to find out(predicted) secondary structure/classification for all of them (so ~6K queries and doing this by hand on numerous ...
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Mitotic chromosome condensation screen

If I want to screen for mutant yeast (S. cerevisiae) colonies that have defects in mitotic chromosome condensation, can I screen for colonies arrested in Mid-M phase? Would that work?
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Evolution home experiment?

If I were to take bakers yeast and put it in medium of minimal sugar(whatever quantity that would be) and rice(for a source of starch), could the yeast have a "evolutionary leap" and adapt to use the ...
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Genetic tests on S. cerevisiae to determine mutation locus on genes

I am studying the metabolism of galactose in the budding yeast Saccharomyces cerevisiae. After a random mutagenesis screen, several mutant strains were isolated that grow well in glucose but are ...
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1answer
304 views

Can yeast in a primary fermentation of wine be used as a source of yeast for new fermentation?

So if I were to pitch bread yeast, and get to primary fermentation, which as I understand it, is the point at which regular cellular respiration can no longer continue due to a lack of oxygen , which ...
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1answer
941 views

Yeast absorption of different spectra of light

I'd like to monitor the growth of a yeast population by shining light through it and monitoring the change in light that gets through as it grows. I'm not sure if I should use IR, or any particular ...
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56 views

What is the maximal insert length for PCR based homologous recombination in S. cerevisae

I would like to insert a 6 kbp construct, which I have on a plasmid into the genome of S. cerevisiae. This plasmid was originally constructed to integrate at the HIS locus via homologous recombination ...
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764 views

Why not use lactase to aid the break down of lactose when making biofuels?

I did an experiment using nine different substrates (Lactose + lactase, corn, sugar cane, glucose control, wood, grass, no yeast control and yeast control) and observed their fermentation rates. ...
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411 views

How does DNA damage cause ageing in yeast?

As I understand it, in yeast ageing there is daughter cell and mother cell. The daughter cell is has newly "fresh" DNA and mother cell dies after some counts of replication. What happens to the ...
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2answers
114 views

Downloading specific yeast genes in an automated manner?

I have 6 genes of Candida albicans yeast namely orf19.723, orf19.5908, orf19.610, ...
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3answers
3k views

Why would yeast grow but not produce alcohol?

I'm studying for my intro to microbiology test and I have a question asking why a culture of yeast responsible for producing alcohol would be growing, but not producing alcohol? The multiple choice ...
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1answer
777 views

What is the best current understanding of how yeast transformation works?

I would like to get myself up to speed with what is currently known to science about yeast transformation. Specifically, transformation of plasmids and linear DNA fragments. I am particularly ...
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2k views

How does yeast dissolve in sugar?

There's this fascinating "kitchen experiment". Take a "brick" of baker's yeast (not dry yeast), and a couple teaspoons of sugar, put them together in a cup and stir together. There's no doubt both are ...
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152 views

Why does the stability of YAC increase with size?

From Lehninger Principles of Biochemistry : The stability of YAC increases with size (up to a limit). Those with inserts of less than 100000 are gradually lost during mitosis. Why does this ...