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Questions tagged [yeast]

Yeasts are a polyphyletic group of unicellular fungi. Usually the term yeast is used synonymously with baker's yeast - Saccharomyces cerevisiae.

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What does the term "centromere linkage" mean in genetics?

I am currently learning about tetrad/linkage analysis in yeast. I have read two statements similar to this: If two genes are unlinked, but linked to the centromere the distribution will be 50 ...
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What is a "marker-matched" plasmid?

I see this term used a lot in papers and have trouble understanding it. From what I have gleaned it seems they are used during control experiments, perhaps with the goal of making the control plasmids ...
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Explanation for the results of Topoisomerase I treatment of ERCs - Gel Electrophoresis

This part of a 1997 paper published in The Cell (David A. Sinclair & Leonard Guarente, 1997) discusses the presence of extrachromosomal rDNA circles (ERCs) in greater numbers in old mutant yeast ...
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Protecting linear ssDNA inside a yeast cell

This is kind of a multi-part question :) Basically, I want a linear single-stranded polydeoxynucleotide to exist inside an S. cerevisiae cell cytoplasm for a long time without being digested. As far ...
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How can I easily distinguish between eukaryotic and prokaryotic cells using flow cytometry?

I am trying to count populations of cells in a co-culture. One organism is Pichia pastoris and the other organism is a gram-negative bacterium. Pichia stains gram-positive using crystal violet and the ...
Noah Sprent's user avatar
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Sabouraud dextrose agar breaking?

I recently started growing some Geotrichum Candidum (GC) on Sabouraud dextrose agar (SDA) beds. The SDA was poured into sterilized petri dishes. After setting the plates in a cooler (20 C) for a day, ...
neydroydrec's user avatar
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Reliable recipe to make DIY yeast slants

Does anyone have any good ideas on a reliable way to activate basic grocery store yeast? I realize this is a total beginner's question. I've built a small yeast ranch at home with my kid, and we're ...
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How to do manual Saccharomyces tetrad separation with minimal equipment?

I would like to separate the four ascospores from individual asci by manual microscopic manipulation without at micro-manipulator and a basic dissecting microscope capable of 600X total magnification ...
Laney Biomanufacturing's user avatar
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Why there are two types of medium (YPGlu and YPGal) in the *K. lactis* Protein Expression Kit?

I am planning to use the K. lactis Protein Expression Kit from NEB for the expression of certain protein. I am not experienced with working with yeast. I started by reading the manual for this kit and ...
Bramborek's user avatar
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Gene of interest number of copies in transformed K. pastoris

When transforming a K. pastoris strain, is there a way to predict how many copies of the gene will be integrated following the homologous recombination? More specifically is it something that a ...
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Does the process of making a sourdough starter involve Darwinian selection?

In essence - to make a sourdough starter: Put flour and water in a jar, and leave it in a warm place overnight Divide the remaining mixture in half, throw out half, add half as much flour and water ...
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Can viruses stabilize and increase the shelf life of wine and other foods?

When you make wine, you use the yeast Saccharomyces cerevisiae to ferment sugary juices. After you're done, you want to avoid them doing anything by using some chemicals etc. There are also other ...
Umut Özdemir's user avatar
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473 views

What are the best plants to cultivate yeast from?

I know that cultivating yeast from potatoes yields a good brewing yeast and that forms of wheat make good baking yeast, however; I have not seen much information on other useful plant yeast cultures. ...
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Species diversity in cheese and yoghurt?

There have been studies on the number of species on humans and in various other environments. I was wondering how many species including: viruses, bacteria, yeasts, and fungi inhabit ordinary farm ...
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Rice left in water for several months - what biological reactions or growths would occur?

In an attempt to make a hair care product, uncooked white basmati rice was left in an air-tight container half filled with tap water for several months. When opening the container, the smell was ...
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Meaning of "f.a." in the species name

What does "f.a." stand for in the name of the "Wickerhamomyces menglaensis f.a." yeast species mentioned in this article https://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0....
Anton Vasetenkov's user avatar
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Clarification of method notation in yeast cultivation method

I'm reading a paper about yeast culture and in the methods, it says: Then $5$ ml of sterile $M/15\ KH_2PO_4$, was added to the slant, What is the meaning of $M/15$?
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Is it normal to see yeast cells stacked in the Z axis when counting yeast?

I just got started with yeast cell counting and was surprised to observe yeast cells that are at different z axis depths at 400x. That is, by adjusting the focus (not the x/y stage) I can make some ...
Clark Updike's user avatar
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Infecting yeast with viruses

Is there any virus, either natural or engineered, known to infect yeast through mechanisms similar to phages/human viruses, that is, by horizontal contamination mediated by extracellular viral ...
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How is Saccharomyces cerevisiae grown on acetate and ammonium able to produce TCA intermediates?

I am working with a metabolic model of Saccharomyces cerevisiae and I am studying its growth on acetate and ammonia. I am performing Flux Balance Analysis to compute the growth rate and then I am ...
Jorge B.'s user avatar
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How can I estimate the total number of yeast cell in a medium?

So I want to run an experiment with yeast. I want to monitor the population as close as possible in regard to its size. I am aware I could use a colorimeter or such, but I can only get a microscope. ...
Brian Blumberg's user avatar
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Continuous (yeast) culture system satisfying multiple criteria

There are multiple ways yeast cells can be grown in liquid cultures. Batch cultures are easy to prepare but cell density, medium composition and physiology change over time Chemostats allow to ...
Mattia Rovetta's user avatar
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1 answer
3k views

How can I measure the population growth of yeast?

I will soon have to carry out an experiment. I want to estimate the rate of growth of a population of yeast, although I don't know if it is possible to at least estimate the number of yeast bacteria ...
Brian Blumberg's user avatar
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Primer design for PRP24-TAPtag

I have a problem with my proteomics homework. I was asked to design primers used to create a homologous recombination box to create PRP24-TAPtag yeast. I know that homologous regions should be 40-50nt ...
Emilia Chojak's user avatar
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Can a plasmid cause cancer? [closed]

Can a plasmid cause cancer? According to wikipedia, plasmids are normally present in bacteria They (plasmids) are normally present in bacteria , and sometimes in eukaryotic organisms such as yeast [...
Dare to ask-I dnt mind punishm's user avatar
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578 views

The effect of sugar on rate of yeast Respiration

In my experiment, I am investigating the effect of different types of sugar on the rate of respiration of immobilized yeast (yeast trapped in calcium alginate balls). I am looking at glucose, fructose,...
Jainil Shah's user avatar
1 vote
1 answer
36 views

Where do I get an annotation file for yeast genome with ENSEMBL ids?

Even the annotation files hosted on the Ensembl site have systematic names (starting with Y..) instead of ENSEMBL ids (starting with EN..). I bet there would be a place where I can get an annotation ...
user345394's user avatar
1 vote
1 answer
107 views

How to measure the length of mitochondria from z stack fluorescent microscopy image?

I have been working on yeast cells to analyse the effect of DNA damaging agents on mitochondrial structure. I have imaged my culture treated with MMS for a period of 6 hours and while observing the ...
Jyoti Sharma's user avatar
3 votes
1 answer
962 views

Where do kombucha SCOBYs get nitrogen?

Kombucha can be brewed even with tiny amounts of inocculate, such as the little bit of sediment at the bottom of store-bought kombuchas. Over 1-2 weeks, this can grow into an inch thick film covering ...
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What is the practical difference between SC, SD and CSM?

Is there some logic to how SC, SD and CSM yeast dropout media differ from each other? I have encountered these three formulations in Sunrise Science Products' catalog, and it appears that the ...
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Can yeast survive without mitochondria?

Some yeast cells have very low mitochondria copy number (see number of mitochondria in non budding cell, you can count mtDNA). This makes them susceptible to fluctuations, and one can conceive that ...
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Yeast contamination with fungus patch

I am working on yeast mutant and I am observing their sporulation efficiency on agar plates. One of my plates got some dark green small fungus patches. I can't grow yeast again and dump this one with ...
Abhishek Tyagi's user avatar
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92 views

Why do some yeast flocculate on top an other at bottom?

When fermenting beer normally after the fermentation has completed the yeast flocculate and drop to the bottom. However occationally they form a foamy layer on top of the beer. The difference is ...
skyking's user avatar
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Protein expression in K. lactis system

I am trying to express a protein in K. lactis Protein Expression Kit (E1000S - NEB) in the way that the protein is secreted into the medium. I know that I can get some protein but it is not active. As ...
Maria's user avatar
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Yeast transformation plate confluent with growth

I have problem with yeast transformation. I tried to transform pyes3-tryp(my insert inside) into INVsc1 yeast cell by following the protocol in the manual for few times but failed(confluent with ...
att901's user avatar
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3 votes
1 answer
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Is the CMV promoter active in yeast?

I wish to transfect yeast and want to control my efficiency. So I thought to use pEGFP-N1 as control. The EGFP is driven by a CMV promoter. Can yeast read CMV promoters. Thanks Hermann
Hermann Norpois's user avatar
5 votes
1 answer
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Is hydrochloric acid suitable for pickling food?

I was wondering whether it is possible to pickle food using hydrochloric acid (HCl), rather than acetic acid. I am not very familiar with the biology of the fermentation process involved in pickling ...
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1 answer
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How do I improve expression using the GAL1 promoter in S. cerevisiae above the "leaky" level I'm currently seeing?

I am attempting to express GFP in S. cerevisiae using the GAL1 promoter. I always grow an uninduced (in dextrose based SD medium) culture alongside my induced (in galactose based SD medium) culture. ...
DanielleB's user avatar
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1 answer
4k views

Why does yeast react the way it does to water combined with sugar?

I almost wanted to ask this in Cooking SE, but thought it would be better fit here. So I understand than when you mix yeast with sugar and warm water, you get CO2. When using baking powder, it's a ...
hgiesel's user avatar
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2 votes
1 answer
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Pichia pastoris and ornithine

Can Pichia pastoris (species of yeast used for recombinant protein) produce the non-protein amino acid Ornithine? Or does it express ornithine at all?
Fiona's user avatar
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1 answer
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What is the rate of mRNA decay in saccharomyces cerevisiae?

I am just looking for a number whether that is a mean or an estimate. I am having trouble finding this value on papers because the papers mention only the methods for calculating such a value. Is ...
Lucas Alanis's user avatar
1 vote
1 answer
369 views

Yeast Strain with squalene synthase / farnesyltransferase (ERG9) gene deleted

I was interested in obtaining a yeast strain without the enzyme farnesyltransferase (Which catalyses conversion of FPP to Squalene). The Euroscraf database has thousands of yeast strains with ...
curious_cat's user avatar
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Y285 Yeast Strain: Where to obtain from?

Often I see yeast strains mentioned like Y285 or Y301 etc. in papers. Is this some sort of universal notation? (Link to poster: https://ddd.uab.cat/pub/tfg/2014/119349/TFG_narcisferrerledo.pdf) How ...
curious_cat's user avatar
1 vote
1 answer
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Enzyme Inhibitors: Using in vivo in yeast

I find listed on the Sigma-Aldrich site a large list of enzymes & corresponding inhibitors. e.g. For the enzyme squalene synthase the inhibitor listed is "Zaragozic acid A trisodium salt" Would ...
curious_cat's user avatar
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1 answer
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Using PGAL10 promoter to overexpress specific yeast genes: How to?

I was reading a poster about expression boosting for Artemesinin production in yeast and it had this line: PGAL1 and PGAL10 have been used for the overexpression of all genes from the ...
curious_cat's user avatar
4 votes
2 answers
1k views

Earliest to use media after autoclaving?

I am autoclaving media to use later to inoculate yeast. How much time do I have to wait before using the media and adding the culture? Can I use it a few hours after autoclaving?
Kasey's user avatar
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What concentration of sugar is optimal for baker's yeast fermentation?

I'd like to do an experiment on what concentration of sugar would be best for fastest yeast fermentation. I'm thinking to put some active dry yeast into different concentrations of sugar water and ...
hm527's user avatar
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4 votes
1 answer
766 views

The effects of auto-brewery syndrome

Why are some people affected so differently by auto-brewery syndrome differently if the syndrome seems to be caused by the single organism saccharomyces cerevisiae? It is known that the syndrome has ...
aspiringSD's user avatar
2 votes
2 answers
102 views

identifying which SNPs sit in TFBS (Yeast)

i have a set of ~11k SNPs for Saccharomyces cerevisiae, baker's yeast and i would like to identify which ones of these sit in transcription factor binding sites and if they do information on the ...
stas g's user avatar
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3 votes
1 answer
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Relationship between turgor pressure and osmotic pressure?

I would like to know if there is a relationship between osmotic pressure (inside and outside of a cell) and turgor pressure. If so, is there a way to formalize it mathematically? Thank you in advance,...
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