Questions tagged [yeast]
Yeasts are a polyphyletic group of unicellular fungi. Usually the term yeast is used synonymously with baker's yeast - Saccharomyces cerevisiae.
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How can I easily distinguish between eukaryotic and prokaryotic cells using flow cytometry?
I am trying to count populations of cells in a co-culture. One organism is Pichia pastoris and the other organism is a gram-negative bacterium. Pichia stains gram-positive using crystal violet and the ...
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Protecting linear ssDNA inside a yeast cell
This is kind of a multi-part question :)
Basically, I want a linear single-stranded polydeoxynucleotide to exist inside an S. cerevisiae cell cytoplasm for a long time without being digested. As far ...
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What is the maximal insert length for PCR based homologous recombination in S. cerevisae
I would like to insert a 6 kbp construct, which I have on a plasmid into the genome of S. cerevisiae. This plasmid was originally constructed to integrate at the HIS locus via homologous recombination ...
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Why not use lactase to aid the break down of lactose when making biofuels?
I did an experiment using nine different substrates (Lactose + lactase, corn, sugar cane, glucose control, wood, grass, no yeast control and yeast control) and observed their fermentation rates. ...
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What does the term "centromere linkage" mean in genetics?
I am currently learning about tetrad/linkage analysis in yeast. I have read two statements similar to this:
If two genes are unlinked, but linked to the centromere the distribution will be 50 ...
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Explanation for the results of Topoisomerase I treatment of ERCs - Gel Electrophoresis
This part of a 1997 paper published in The Cell (David A. Sinclair & Leonard Guarente, 1997) discusses the presence of extrachromosomal rDNA circles (ERCs) in greater numbers in old mutant yeast ...
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Species diversity in cheese and yoghurt?
There have been studies on the number of species on humans and in various other environments. I was wondering how many species including: viruses, bacteria, yeasts, and fungi inhabit ordinary farm ...
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Infecting yeast with viruses
Is there any virus, either natural or engineered, known to infect yeast through mechanisms similar to phages/human viruses, that is, by horizontal contamination mediated by extracellular viral ...
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How is Saccharomyces cerevisiae grown on acetate and ammonium able to produce TCA intermediates?
I am working with a metabolic model of Saccharomyces cerevisiae and I am studying its growth on acetate and ammonia. I am performing Flux Balance Analysis to compute the growth rate and then I am ...
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Primer design for PRP24-TAPtag
I have a problem with my proteomics homework. I was asked to design primers used to create a homologous recombination box to create PRP24-TAPtag yeast. I know that homologous regions should be 40-50nt ...
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The effect of sugar on rate of yeast Respiration
In my experiment, I am investigating the effect of different types of sugar on the rate of respiration of immobilized yeast (yeast trapped in calcium alginate balls). I am looking at glucose, fructose,...
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What is the practical difference between SC, SD and CSM?
Is there some logic to how SC, SD and CSM yeast dropout media differ from each other? I have encountered these three formulations in Sunrise Science Products' catalog, and it appears that the ...
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Why do some yeast flocculate on top an other at bottom?
When fermenting beer normally after the fermentation has completed the yeast flocculate and drop to the bottom. However occationally they form a foamy layer on top of the beer.
The difference is ...
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Protein expression in K. lactis system
I am trying to express a protein in K. lactis Protein Expression Kit (E1000S - NEB) in the way that the protein is secreted into the medium. I know that I can get some protein but it is not active. As ...
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Yeast transformation plate confluent with growth
I have problem with yeast transformation. I tried to transform pyes3-tryp(my insert inside) into INVsc1 yeast cell by following the protocol in the manual for few times but failed(confluent with ...
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Mass Spectrometry Artefacts: R-deamidation / Oxidation?
Context: I am a student-researcher. I expressed GST-tagged protein (Pyruvate Kinase) in the yeast Saccharomyces cerevisiae on synthetic drop-out media. I then purified the GST-tagged protein, ran it ...
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How to do manual Saccharomyces tetrad separation with minimal equipment?
I would like to separate the four ascospores from individual asci by manual microscopic manipulation without at micro-manipulator and a basic dissecting microscope capable of 600X total magnification ...
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Why there are two types of medium (YPGlu and YPGal) in the *K. lactis* Protein Expression Kit?
I am planning to use the K. lactis Protein Expression Kit from NEB for the expression of certain protein. I am not experienced with working with yeast. I started by reading the manual for this kit and ...
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Clarification of method notation in yeast cultivation method
I'm reading a paper about yeast culture and in the methods, it says:
Then $5$ ml of sterile $M/15\ KH_2PO_4$, was added to the slant,
What is the meaning of $M/15$?
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Is it normal to see yeast cells stacked in the Z axis when counting yeast?
I just got started with yeast cell counting and was surprised to observe yeast cells that are at different z axis depths at 400x. That is, by adjusting the focus (not the x/y stage) I can make some ...
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Y285 Yeast Strain: Where to obtain from?
Often I see yeast strains mentioned like Y285 or Y301 etc. in papers. Is this some sort of universal notation? (Link to poster: https://ddd.uab.cat/pub/tfg/2014/119349/TFG_narcisferrerledo.pdf)
How ...
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Mitotic chromosome condensation screen
If I want to screen for mutant yeast (S. cerevisiae) colonies that have defects in mitotic chromosome condensation, can I screen for colonies arrested in Mid-M phase? Would that work?
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Need help choosing a plasmid that induces increased trehalose and glycerol production in yeast
I wanted to know how I should go about choosing a plasmid that increases trehalose and glycerol production in yeast or if anyone had any ideas on suitable plasmids.
