Questions tagged [yeast]

Yeasts are a polyphyletic group of unicellular fungi. Usually the term yeast is used synonymously with baker's yeast - Saccharomyces cerevisiae.

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What is the maximal insert length for PCR based homologous recombination in S. cerevisae

I would like to insert a 6 kbp construct, which I have on a plasmid into the genome of S. cerevisiae. This plasmid was originally constructed to integrate at the HIS locus via homologous recombination ...
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796 views

Why not use lactase to aid the break down of lactose when making biofuels?

I did an experiment using nine different substrates (Lactose + lactase, corn, sugar cane, glucose control, wood, grass, no yeast control and yeast control) and observed their fermentation rates. ...
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Species diversity in cheese and yoghurt?

There have been studies on the number of species on humans and in various other environments. I was wondering how many species including: viruses, bacteria, yeasts, and fungi inhabit ordinary farm ...
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Infecting yeast with viruses

Is there any virus, either natural or engineered, known to infect yeast through mechanisms similar to phages/human viruses, that is, by horizontal contamination mediated by extracellular viral ...
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How is Saccharomyces cerevisiae grown on acetate and ammonium able to produce TCA intermediates?

I am working with a metabolic model of Saccharomyces cerevisiae and I am studying its growth on acetate and ammonia. I am performing Flux Balance Analysis to compute the growth rate and then I am ...
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Primer design for PRP24-TAPtag

I have a problem with my proteomics homework. I was asked to design primers used to create a homologous recombination box to create PRP24-TAPtag yeast. I know that homologous regions should be 40-50nt ...
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323 views

The effect of sugar on rate of yeast Respiration

In my experiment, I am investigating the effect of different types of sugar on the rate of respiration of immobilized yeast (yeast trapped in calcium alginate balls). I am looking at glucose, fructose,...
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254 views

What is the practical difference between SC, SD and CSM?

Is there some logic to how SC, SD and CSM yeast dropout media differ from each other? I have encountered these three formulations in Sunrise Science Products' catalog, and it appears that the ...
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61 views

Why do some yeast flocculate on top an other at bottom?

When fermenting beer normally after the fermentation has completed the yeast flocculate and drop to the bottom. However occationally they form a foamy layer on top of the beer. The difference is ...
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57 views

Protein expression in K. lactis system

I am trying to express a protein in K. lactis Protein Expression Kit (E1000S - NEB) in the way that the protein is secreted into the medium. I know that I can get some protein but it is not active. As ...
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87 views

Yeast transformation plate confluent with growth

I have problem with yeast transformation. I tried to transform pyes3-tryp(my insert inside) into INVsc1 yeast cell by following the protocol in the manual for few times but failed(confluent with ...
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71 views

Genetic tests on S. cerevisiae to determine mutation locus on genes

I am studying the metabolism of galactose in the budding yeast Saccharomyces cerevisiae. After a random mutagenesis screen, several mutant strains were isolated that grow well in glucose but are ...
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19 views

Clarification of method notation in yeast cultivation method

I'm reading a paper about yeast culture and in the methods, it says: Then $5$ ml of sterile $M/15\ KH_2PO_4$, was added to the slant, What is the meaning of $M/15$?
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26 views

Yeast integrating vectors: where to cut?

I understand that yeast integrating plasmids (YIps) must be linearized in order to promote homology-directed recombination. However I don't quite understand where. In the end, the external regions of ...
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32 views

Is it normal to see yeast cells stacked in the Z axis when counting yeast?

I just got started with yeast cell counting and was surprised to observe yeast cells that are at different z axis depths at 400x. That is, by adjusting the focus (not the x/y stage) I can make some ...
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41 views

Y285 Yeast Strain: Where to obtain from?

Often I see yeast strains mentioned like Y285 or Y301 etc. in papers. Is this some sort of universal notation? (Link to poster: https://ddd.uab.cat/pub/tfg/2014/119349/TFG_narcisferrerledo.pdf) How ...
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Mitotic chromosome condensation screen

If I want to screen for mutant yeast (S. cerevisiae) colonies that have defects in mitotic chromosome condensation, can I screen for colonies arrested in Mid-M phase? Would that work?