In RNA sequencing, the RNA is fragmented, DNA is synthesized complementary to the RNA fragments, which is followed by a complementary strand synthesis. Fragmentation can be done after the cDNA synthesis too. This DNA is then amplified to form a cluster that is sequenced. Most Next-Gen sequencing approaches sequence a short segment of the DNA (it has improved with experimental and technical optimization). A read refers to the sequence of a cluster that is obtained after the end of the sequencing process which is ultimately the sequence of a section of a unique fragment. If an RNA is expressed in high copies then there will be more reads coming from it; reads can be redundant as well. <br> <br> ---- ![enter image description here][1] [Courtesy](http://home.cc.umanitoba.ca/~zhangx39/PLNT7690/presentation/presentation.html) [1]: https://i.sstatic.net/zdRql.jpg