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The study of the molecular processes underlying life.

5 votes

What is the importance of urea in mass spectrometry?

I think that the OP was asking about relevance of using urea with respect to the FASP method. In the FASP method, the primary denaturant is SDS . Protein are denatured with a ~4% SDS solution (buffere …
ktyagi's user avatar
  • 261
0 votes

What is the best way to analyze non-quantitative mass spec hits from an immunoprecipitation ...

You can look for the most abundant proteins in your sample. Although, as you say, the LC-MS analysis of your IP was non-quantitative, you can still try to get some quantitative estimation. To elaborat …
ktyagi's user avatar
  • 261