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Metabolism is the set of defined biochemical transformations occurring within the cells of living organisms.

2 votes
0 answers
158 views

Regulation of Cra protein level in E coli

Catabolite Activator/Repressor, Cra protein (formerly known as Fructure Repressor FruR) plays a significant role in central carbon metabolism of E coli. … I found enough literature on how Cra plays a regulatory role in metabolism. …
DurgaDatta's user avatar
2 votes
1 answer
4k views

Anaerobic respiration choice in E. coli

Under anaerobic conditions E. coli has two options to generate ATP: fermentation (substrate-level phosphorylation), and respiration (proton gradient, chemisomotic phosphorylation). Which is favored? W …
DurgaDatta's user avatar
5 votes
3 answers
5k views

What is the mechanism of oxygen uptake in E. coli?

Are there bacteria which cannot consume oxygen for metabolism, but when exposed to oxygen they don't die, rather simply discard the oxygen and carry out fermentation? … In a sense, they are strict anaerobe (their mode of metabolism is anaerobic), but they can still survive in presence of oxygen in environment. …
DurgaDatta's user avatar
5 votes
2 answers
1k views

Tracking of oxygen molecules in glucose oxidation

I am interested in this question because the central metabolism can be understood as a electron transfer process where glucose is donor and oxygen is acceptor. …
DurgaDatta's user avatar
4 votes
1 answer
64 views

Need for two oxygen sensors in E. coli

E. coli has two oxygen sensors: FNR (fumarate-nitrate reductase) and ArcBA (Anoxic Redox Control, two component control systems). FNR directly senses the oxygen, while the interaction of ArcB with oxy …
DurgaDatta's user avatar
4 votes
1 answer
3k views

Consumption of NAD+ in glycolysis

Out of 10 steps in glycolysis, only one reaction- Glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (PGP), uses NAD+ and thereby producing NADH. Furthermore, this very step is solely respons …
DurgaDatta's user avatar