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Results tagged with biotechnology
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user 22392
Biotechnology is a field of biology which utilizes biological systems like microorganisms to produce valuable products, such as biofuels, drugs, and vaccines.
5
votes
Plasmids for high school students?
I'd suggest looking at the Terms and Conditions for nonprofit use, here is a screenshot of where it is on the webpage:
Look at this form that they link to for legal understanding of what they mean:
…
- 10.8k
2
votes
Accepted
How many non-pairing bases can a PCR primer have in directed mutagenesis?
It depends to some extent on experimental parameters. For example, how long can your oligos be? This old publication has a functional site-directed mutagenesis primer with 9 mismatches, but the oligo …
- 10.8k
4
votes
Accepted
What is the minimum Ct value that you would consider indicates no gene expression?
Can't you just do a negative control and use that to calibrate?
Alternately, run the DNA from your PCR on a gel with a positive control indicating expression. Literally zero expression should mean tha …
- 10.8k
3
votes
Is there potential to modify GM crops to inhibit their reproduction with non-GM crops?
I can imagine a couple of different ways to do it. One would be "terminator" seeds of the kind that you mention. Another might be a gene drive style system to make GM-wild hybrids sterile.
I would arg …
- 10.8k
3
votes
Why is random protein selection not used more often in biotech to create proteins with desir...
Think about it from a numbers perspective. Briefly, $10^{13}$ is a very small number in the combinatorics of protein sequence space.
There are $20^{100} = 1.267651*10^{130}$ possible peptides of 100 a …
- 10.8k
1
vote
Is CRISPR being utilized when scientists use the CRISPR/Cas9 system to edit genes?
Figure 1. Process of bacterial immunity mediated by CRISPR/Cas9. Scissors are Cas9 enzyme, black diamonds are CRISPR elements in DNA.
As suggested in the comment, there are some subtle differences he …
- 10.8k