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Results tagged with pcr
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user 43530
Polymerase Chain Reaction is a method of replicating DNA exponentially from even a single molecule.
0
votes
PCR not working
MgCl2 can also be the reason.i have tried titers of MgCl2 1.5 ,2,2.5,3 with all other conditions same and it worked
-1
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Choosing PCR conditions
According to my knowledge the main thing in annealing temperature in PCR
Primers annealing temperature should be selected by keeping in 5° less than the lower melting temperature of primers
Secondly … MgCl2 working Concentration also plays a major role in PCR optimization …
-1
votes
Polymerase Chain Reaction Questions
Beside helping Taq to add dntps to the growing strand
Primers minimize the risk of mishybirdization as they are designed specifically for a specific region of DNA