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Results tagged with pcr
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user 59594
Polymerase Chain Reaction is a method of replicating DNA exponentially from even a single molecule.
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High concentration of primer in PCR
The optimum concentration of primers in a PCR reaction is between 0.1 and 0.5 µM. For most applications 0.2 µM suffices. … Using very high concentration of primers can be troublesome as it can cause your primers to bind to partially complementary sites resulting in non-specific PCR products. …
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Humic Acid and PCR
Quite a few papers claim humic acid is an inhibitor of PCR reactions. … I understand this is true when working with soil microbes, but how does it qualify to be a PCR inhibitor in general (i.e when not working with soil micorbes)? …
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