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Synthetic biology is an approach to engineer biology. Core to the discipline is genetic engineering, which is made more akin to engineering by adding the levels of standardization, automation, and abstraction.

6 votes
1 answer
925 views

How can I convert plate reader measurements of Pichia pastoris OD to cells per ml?

I am performing experiments with the yeast Pichia pastoris where it will be beneficial to calculate product yield per cell. I plan to use a plate reader to measure OD600 of my cultures and then someho …
Noah Sprent's user avatar
11 votes
Accepted

Why is the start of my coding sequence ATG and not TAC?

This image from the Kahn Academy article 'Overview of transcription' might help: Essentially, the sense/coding strand of the DNA encodes the sequence that is transcribed. The RNA polymerase binds to …
Noah Sprent's user avatar