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The science of collecting and analyzing complex biological data such as genetic codes using informatics.

-2 votes

What E-value BLAST cut-off is best?

The e-value is required to have confidence in the hit, along with hit. Generally, an e-value of e-4 is preferred because, this cutoff is found to be sufficient enough by blast to confirm a hit as homo …
Prakki Rama's user avatar
2 votes
Accepted

To what extent is it possible to understand if a bacteria can produce a protein? (in silico ...

Your problem will finally boil down to searching your sequence in the Blast databases. Performing Blast seems to be probably the best way to find out if your bacteria has that specific protein express …
Prakki Rama's user avatar
1 vote

What is the close and related genome used for in Gene models?

Why a related genome helps: 1) Alignment of the reads first and assemble next. 2) The gene-space is already predefined ( the genes and their co-ordinates are already known), so if your assembly is f …
Prakki Rama's user avatar
1 vote

About the quality of microsatellites

Try running software like Censor. If checks in the reference repeat databases and detects microsatellites. So, you can most probably be sure of the microsatellites generated. Moreover, the microsatell …
Prakki Rama's user avatar
1 vote
Accepted

is there a way to do batch download for all gene pathways?

Try using KEGG REST. http://rest.kegg.jp/link/pathway/hsa ## list of all genes and pathways in Humans
Prakki Rama's user avatar