New answers tagged antibody
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From Wikipedia: "The name is derived from the city of origin (Kiel, Germany) and the number of the original clone in the 96-well plate".
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Yes. Samples for SDS-PAGE are mixed with a loading buffer containing SDS (final concentration of SDS is usually 2%). Generally, the SDS will disrupt protein-protein interactions, even before boiling. Reducing agent (usually DTT or b-mercaptoethanol) is added to disrupt disulfide bonds.
See diagram here.
There are, however, exceptions. Some protein complexes/...
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