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Does inoculum volume affect spread plate outcome?

To add to the answer by @MikeyC . The spread plate technique for calculation of colony forming units has volume plated as a variable: CFU/ml = (number of colonies x dilution factor) / volume of ...
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Does inoculum volume affect spread plate outcome?

Theoretically, for CFU determination the volume of the inoculum shouldn't matter as long as you plate a dilution series and find the plate with a countable number of colonies. It also shouldn't matter ...
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Is there any alternative to peptone and beef or yeast extract?

There is an article about using cheap substitutes for peptone, one of the main ingredients of culture medium as a nitrogen-source. The authors used coconut cake (meal), ground-nut or gingelly cake ...
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