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I worked with Bacillus subtilis WB800 and just used 50% glycerol / 50% LB. I imagine the same would work fine with wild-type. I would prepare the glycerol/LB mixture and autoclave it then create the stocks from log-phase (approximately) plate cultures. This method was sufficient for my application (protein expression).


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Autoclave is standard, but bleach can be used. In your case, you'll want all the culture media and dish surfaces to have contact with the diluted bleach solution. I would prepare diluted liquid bleach as described in the link below and pour some in a plastic wash tub to a depth where you can put all your petri dishes etc in and have them fully submerged. Let ...


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Minor correction first: Bacterial endospores don't result from one cell differentiating into a different cell type. An endospore is not a cell type, but rather a protein structure that first forms inside of a mother cell, carrying all of the necessary machinery and genetic material to remain viable outside of the mother cell and eventually form a new cell ...


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