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Goldman equation is not applicable in this case. If you have a non-permeable membrane, all the permeabilities of the ionic species ($P_x$) are zero, which leads to an indeterminate form of the argument of the logarithm in the Goldman equation. The logarithm does not equate to $0$. Consequently, Goldman equation cannot be used to find the membrane potential ($...


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The differences in sedimentation speed between the differently marked DNA molecules are not lare enough to allow separation in a low-density medium such as saline, or distilled water. As mentioned by @Polypipe Wrangler DNA will always completely sediment to the bottom of the tube. To improve separation a so called isopycnic centrifugation is performed. ...


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