New answers tagged biotechnology
2
The double-stranded DNA fragments are fractionated by size during the agarose gel electrophoresis. Prior to the so-called “blotting” step, the gel is briefly soaked in a solution of NaOH, which has a relatively high pH. Under these conditions, double-stranded DNA will denature, or “melt” into two single strands. This is the material that is “wicked” or “...
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This graph from the Broad's Opinionome blog (ugh) is somewhat more annotated:
As noted elsewhere, the precipitous drop in 2007 is almost certainly due to maturing next-gen sequencing (NGS), in particular Illumina. Illumina acquired Solexa in 2007, which offered gigabase-level sequencing ability. In 2014, President of Illumina Francis de Souza essentially ...
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It would make the result at least a little ambiguous. How would you establish with high confidence that the offspring was the results of your implanted cloned ovum and not a naturally fertilized one? DNA testing would have to be quite extensive to establish that offspring was a clone and not the result of an ordinary pregnancy. If you use a surrogate mother, ...
3
From your question I suspect that you are thinking of DNA as being a very rigid molecule — it isn't.
Double stranded DNA is reasonably flexible and a useful mental model would be to think of this as being like tying the ends of two pieces of thin wire together to make a circle.
Double stranded DNA can make a closed circles smaller than 250 bp long1,2 and ...
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I'm not 100% sure, but I think Illumina launched their HiSeq machine around that time, massively increasing the sequencing output in one run. That dip might be related to that.
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The graph is in contradiction with the data in the Excel spreadsheet the same webpage provides (https://www.genome.gov/sites/default/files/media/files/2019-06/Sequencing_Cost_Data_Table_Feb2019.xls (mirror)), which I plotted below:
https://en.wikipedia.org/w/index.php?title=\$1,000_genome&oldid=902062326#Commercial_efforts to some of the dates on the ...
-1
Crossing over is an event that occurs during meiosis, which produces the gametes. This crossing over occurs between 2 homologous chromosomes, which are both from the parent. Hence, even though crossing over occurs, the recombinant chromosome that is passed down to the child will contain an allele that matches with the allele on one of the parent's ...
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After completing my tests, I have decided to come back to answer my own question.
A Nanodrop machine has served my purpose quite efficiently, as far as I can judge. I used have used it to evaluate the degree of purity of a natural alkaloid extract from insects, using synthetic alkaloid analogues as controls. See our published paper discussing the method ...
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