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2 votes
Accepted

How would one go about isolating a specific mRNA out of a pool of others?

First of all you need to figure out what your goal is: an expression vector (i.e. plasmid) which will drive expression of a gene in bacteria (let's say E.coli), and your gene's coding sequence needs ...
Armatus's user avatar
  • 7,670
2 votes

How is an RT-PCR representative of the RNA contents in a cell?

It isn’t really clear exactly what the questioner is asking, but… ...one way of thinking about this is to ask how likely is it that a low-abundance RNA will be absent from the sample taken from the ...
Alan Boyd's user avatar
  • 22.8k
2 votes

Longest transcripts without isoforms

To R! Download the most comprehensive GTF file from Gencode for mouse. Do the following in R (I'll put comments in the code so you can hopefully follow along): ...
Devon Ryan's user avatar
1 vote

Minimum amount of RNA for cDNA conversion and qPCR

Two answers follow: Technical experience of many: it should be no problem to have the same protocol with a twice-lower starting concentration, without making any adjustments whatsoever. The suggested ...
S Pr's user avatar
  • 6,272

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