6

Most guidelines for HeLa (and most cells of human origin) say they should be kept at a BSL2 level. For example, from a 2007 publication in Applied Biosafety: Work with cell cultures from human or primate origin should generally be performed under BSL2 conditions. Containment level 1 may be considered if all manipulations occur in a Type II biosafety ...


3

In the mentioned study, they tried to support the evidence from earlier studies in which caffeine consumption was associated with skin aging and slow wound healing: Our research confirms our earlier observations that caffeine may have an adverse effect on the wound healing process, as well as on the aging process, of the human skin. ...and by "...


2

How can one relate in vitro studies of caffeine (dose expressed as concentration) to dietary intake of caffeine (dose in mass)? Caffeine rapidly diffuses throughout the body's water, both intra and extracellular. Therefore, since the caffeine will be relatively evenly distributed, you can calculate the amount of caffeine needed to reach a given molar ...


1

I'm not sure the relationship you are trying to build between EMT and cancer cells. Yes some "healthy" cells undergoing EMT could (due to specific underlying mutations) result in cancer cells but such a phenomenon is not a "hard and fast" rule. The majority of ctDNA would likely be (as you suggest) from lysed cells, however, should mutations occur that ...


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