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There are no formal definitions accepted in science for ambiguous phrases such “protein expression”. If you wish to know what a particular author who uses such a shorthand phrase means you need to examine the context. If you wish to write scientific reports that are comprehensible to others you must express yourself precisely, defining any shorthand phrases ...


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The Thermo Fisher definition is more explicitly accurate, although one could argue that the term "production" in the NCI definition implies regulation and post-translational modification (PTMs) as well. Either way, protein expression definitely includes regulation. Typically, when you measure protein expression, such as via Western blot, you are ...


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Adding a third viewpoint, I'm primarily an electrophysiologist in neuroscience. In my corner of the world, I have to say we can be a bit sloppy about terminology from molecular biology. There might be more precise meanings intended in other areas, but when someone writes "protein expression" I typically translate this roughly to "we measured ...


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GSK3 is interesting because as a kinase, it is itself regulated by its phosphorylation status (particularly by Akt in neurons). Therefore, more protein does not necessarily mean more activity. For example from E13.5 to E15.5 GSK3 levels do not change but activity steadily increases. Hence if you increase GSK3 levels in neurons, it may not mean that ...


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I would suggest that the preferable term depends on whether you are talking about the cells or talking about the modifications. In other words, I would refer to the NCAM2 expression package as the "heterologous expression system", which is then integrated into the CHO platform in order to create a "heterologous cell model." My reason for ...


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Generally speaking, the proper way to quantify a western blot is to normalize to a loading control such as Actin or GAPDH. In this case it would be (pGSK3/Actin)/(GSK3/Actin) as total GSK3 is not a loading control. A loading control is to protein that is accepted as unvarying in concentration across multiple samples if the same protein amoun is used, and to ...


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Please consider this "answer" as an enlarged comment. I can't answer your question, but it's possible that my speculations or considerations could be helpful in your particular situation, in the early phase of building a model. First of all, I doubt that general formulas that link cell-volume to ER-volume of different cell-types can exist. ...


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Plant leaves develop from leaf primordium or meristem tissue (this tissue has special properties that allow it to continue dividing to make more more cells, i.e. stem cells - not stem cells like cells in the stem but the technical term 'stem cells). Because of the hormone balances to maintain this division property the meristem tissue will remain close to ...


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At least some plants have cellulases which can be used to cleave primers from mature cellulose chains. Cellulose synthesis requires chain initiation and elongation (the two processes are separate). CesA glucosyltransferase initiates cellulose polymerization using a steroid primer and UDP-glucose. Cellulose synthase utilizes UDP-D-glucose precursors to ...


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