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11 votes

Bacterial cells disappearing from culture with time

That sounds like phage. The pattern of initial growth followed by catastrophic death with some cell debris is classic for induction and lysis. Have you observed anything strange on plates? You could ...
ksdjnf's user avatar
  • 186
3 votes
Accepted

Where can I find a video of human cells reacting to cold water vs warm?

You can't see these changes easily under a microscope. It is a challenge to add heat or cold to cells on a microscope in a manner that you might see, unless you have the right equipment. You might ...
bob1's user avatar
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3 votes
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How much effort is it to establish a cytotox assay for cancer cell lines against a small number of possible compounds?

The answer to this is, of course, "it depends". There are quite a few factors at play here. As you don't seem to be experienced at cell culture yet, I strongly recommend that you find ...
bob1's user avatar
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2 votes
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Cell viability assay: Problems with MTT assay in the solubilization step

The easy answer is to use acidified SDS 10 Percent in 0,01 N HCl. I added this directly, to my media and did not have any problems with crystal removal anymore. The acid will remove the coloring of ...
raptorlane's user avatar
2 votes
Accepted

Why does my cell subculture have these black dots like appearance?

I'm thinking you are very new to cell culture - your first step should be to ask people in your lab. You have a couple of problems here - it takes time for cells to settle and attach and start to ...
bob1's user avatar
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2 votes

Can a non-pure culture be used for reliable rapid phenotypic diagnostic tests?

Isn't the answer in the quote you provided from Manual of Standards for Diagnostic Tests and Vaccines? The results won't be reliable. Having pure cultures by re-streaking the ones you suspect and only ...
bob1's user avatar
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1 vote

Induction of IFN-beta in HEK293T

HEK293 cells (whatever the variety) are not an excellent substitute for PBMCs, as they are from a completely different cell lineage and behave very differently in a lot of ways. However, if the paper ...
MattDMo's user avatar
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1 vote

What kind of bubble this´╝č

Late stage apoptosis. Specifically what you are seeing here is cells that have undergone the blebbing stage and are releasing or have released apoptotic bodies.
bob1's user avatar
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