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2 votes

How are single cells sorted in an scRNA-Seq protocol by FACS?

we don't know the baseline of CD markers' expression levels yet? There are standard settings that can be used between runs that will give consistent results from one sample to the next. There are ...
iayork's user avatar
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2 votes

How are single cells sorted in an scRNA-Seq protocol by FACS?

I will append the previous sentence in your protocol, and put what might help you in bold: To obtain B cells, NK cells and monocytes, a splenocyte suspension was stained with PE-Cy7-...
S Pr's user avatar
  • 6,262
1 vote
Accepted

Flow cytometry data Analysis

Disclaimer: Assumes you know how flow cytometry works. You've done a flow-based AnnexinV apoptosis assay here. Annexin A5 (AnnexinV) protein binds to phosphatidylserine (PS), which is typically in ...
CKM's user avatar
  • 8,109

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