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2 votes

Questions about ChIP-seq reads and MACS

"ChIP-Seq tags represent only the ends of the ChIP fragments, instead of precise protein-DNA binding site" (Background). (Q2) Therefore, shouldn't it be that we get reads mapping to either ...
Maximilian Press's user avatar
2 votes

What are the effects of buffer on genomic DNA shearing by sonication?

Could you use a spun column to exchange the buffer? Some sort of gel filtration column; equilibrate column in TE; add your DNA sample, spin through excluded DNA. TKMC components will be included in ...
Alan Boyd's user avatar
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1 vote

Interpreting ChIP-qPCR or ChIP-seq

Protein binding to DNA is not bivalent. ChIP is enriching for DNA to which your protein of interest is bound, but that protein may bind certain sites throughout the genome more strongly and frequently ...
Jared Andrews's user avatar

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