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21 votes
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Can a human be made with ovum but without sperm?

Nice question! It is actually very difficult to do so because humans, obviously, are far more complex than some animal species which naturally show parthenogenesis. Just to mention, there are female-...
another 'Homo sapien''s user avatar
12 votes
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Are drones from one honey bee queen all clones?

The queen is diploid; the drones are haploid. Meaning the queen has two copies of each chromosome, and the drones have only one. So for each gene, the queen has two copies and passes one of those ...
Oosaka's user avatar
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12 votes
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What if target DNA doesn’t have restriction sites

If you're synthesizing the insert then you just design it with restriction sites that are compatible to the vector. Otherwise, you can design PCR primers that have restriction sites in them. That way ...
Catherine's user avatar
  • 191
6 votes
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Is it Theoretically Possible to Create a Male clone from the donor Cell of a Female?

So, i want to know if the sex of the resulting clone is determined by the sex of the donor animal. Generally yes for mammals and birds. In animals where the sex is determined by sex chromosomes, ...
JayCkat's user avatar
  • 2,932
6 votes

Plating 96-well bacterial transformations

I think your approach very much depends on how essential it is that you get discrete single colonies, and if this is a one off or a regular process. To preface, similar to what Maximilian Press said, ...
Matt's user avatar
  • 61
6 votes
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If we cloned an extinct animal, what would become of its gut biome?

The gut biome would be populated from whatever the environment was of the newly "cloned" animal. Presumably the baby animal would be born by birth from a live host mother, which would be the ...
Maximilian Press's user avatar
5 votes

"Antibiotic resistance" equivalent in archaea for selection during cloning

Obviously, one can't use antibiotic resistance since they are not bacteria. This is not so obvious. Some common antibiotics are active against archaea.1 The Halohandbook, an indispensable document ...
acvill's user avatar
  • 8,326
5 votes

Why we need an egg cell donor and a surrogate mother in an organism cloning process?

Could a whole set of artificial organism-cloning process only be operated on a somatic nuclear donor? No, this would not be possible. Single-cell reprogramming would enable you to reset the potency ...
rlluc's user avatar
  • 134
5 votes

Production of plant derivatives using genetic engineered micro-organisms

In principle, any biological product should be able to be developed through microbial synthesis, with the appropriate choice of chassis. Indeed, this was the goal of the DARPA "1000 Molecules&...
jakebeal's user avatar
  • 7,007
4 votes
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Misuse of DNA leaks to create your clones, or children

As @iayork mentions in their comment, creating a human clone from a sequenced genome is still in the realm of science fiction. Also, DNA testing companies like MyHeritage don't sequence the client's ...
Charles E. Grant's user avatar
4 votes

Why is cDNA usually lacking in terminal sequences of the template mRNA?

Traditionally cDNA cloning used an oligo(dT) primer that binds to the 3′ poly(A) tail of a mRNA and extends towards the 5′ end of the mRNA. What makes it difficult for the reverse transcriptase to ...
David's user avatar
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3 votes
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If a woman was fertilized with her own DNA, would it be her clone?

1) In principle yes, you could in theory use micro injection to inject haploid DNA into the egg. I don't think this has been used in practice though. However Dolly the sheep was created by exchanging ...
Jeppe Nielsen's user avatar
3 votes

How can seedless fruits not be GMO?

GMO stands for Genetically Modified Organism. Grafting a plant to a rootstock of another plant is a form of plant husbandry -- a farming technique -- but this isn't changing the DNA code of either ...
Stephanie Waters's user avatar
3 votes

Isolating gene of interest from a large strand of DNA

Assuming that you know exactly where in the full DNA strand the gene of interest is located, it is fairly easy to isolate the gene. There are several restriction enzymes you can use, it's just a ...
Astrolamb's user avatar
  • 511
3 votes
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Genetic information storage and the extent to which it controls appearance/structure

The common phrase I've heard is "DNA isn't a blueprint, it's a recipe". More specifically, the nucleotides in DNA correspond to which proteins get made (coding DNA) and when and where (regulatory DNA)....
Oosaka's user avatar
  • 3,253
3 votes
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Alkaline Phosphatase and Ligase Protocol for Cloning

DNA ligase catalyzes the formation of a bond between 3’-OH and 5’-PO4 of its nucleic acid substrate. (See Lehnman, 1974) The ligation bond will be formed when the proper ends are close together, ...
z1273's user avatar
  • 556
3 votes
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Production of plant derivatives using genetic engineered micro-organisms

General Considerations The question asks specifically why certain plant products are not produced commercially in genetically modified micro-organisms. There are some general reasons, illustrated in ...
David's user avatar
  • 26.3k
3 votes
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T-vector creation

Personally I would use Taq DNA polymerase to A overhang my inserts, especially if you are PCR amplifying the insert. It leaves an A overhang on the end of all amplicons natively. All you have to do ...
bob1's user avatar
  • 12.5k
3 votes

Using PCR to add the overhangs to gBlocks for NEB HiFi

You could add the overhangs with PCR, then gel purify or PCR purify. I don't think it will pose a big problem as I've done it before and all of the colonies I screened were correct.
Caspase's user avatar
  • 31
3 votes

Hierarchial and shotgun sequencing vs Massively Parallel Sequencing

The construction of sequencing libraries for Illumina, PacBio, ONT, or similar instruments is a large field of methodology that encompasses a variety of different techniques. For background on ...
Maximilian Press's user avatar
3 votes
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Why does plasmid cloning efficiency go down with increased size of insert?

I suspect, but don't have any proof, that it is the physical difficulty of getting both ends ligated to a single strand - think of it like this: Your 10 kb is essentially a long string, ligate one end ...
bob1's user avatar
  • 12.5k
2 votes

Why does the cloned cat, CC, exhibit mosaic fur color?

There are mechanisms through which the X-chromosome that has been turned into a Barr-body can be re-activated. This happens during cloning, but is also seen in some cells that turn into cancer cells. ...
Yuri Robbers's user avatar
2 votes
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Difference between clonal and subclonal mutations

clones means when a cell has the same DNA characteristics as his predecessor so in that case of yours you could say that those cells are clones while for sub-clones I am not that sure, the difference ...
Fation Jesmina Mashi's user avatar
2 votes
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Why doesn’t p53 cause the repair of cellular DNA that has been altered experimentally?

I am no expert in this area, and this answer is only based on a reading of the Wikipedia article on p53, which you should perhaps have read carefully. I welcome edits or correction by persons more ...
David's user avatar
  • 26.3k
2 votes
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When is strict sterile technique necessary? Cloning vs. protein expression

Short answer is it never hurts to practise thorough aseptic technique. My general rule of thumb is, if I'm opening a culture of bacteria (plates, eppendorfs, tubes etc.) I do it under a bunsen - the ...
Joe Healey's user avatar
  • 1,301
2 votes

When is strict sterile technique necessary? Cloning vs. protein expression

For most plasmid preparation, strict sterile technique is not necessary. The antibiotics in the media will select for bacteria which carry a resistant plasmid, so you don't usually have to worry about ...
user137's user avatar
  • 5,348
2 votes
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Can you make sperm and eggs from the same person using in vitro gametogenesis?

At present (early 2017) the answer is No. The trick to make spermatid like cells (ie they are not real sperm cells and need to be injected into an oocyte to accomplish fertilization) from ES and iPS ...
JayCkat's user avatar
  • 2,932
2 votes

Are clones less likely to be fertile?

Cloned animals, at least in the first generation, are often subfertile and have lower viability than their progenitors. The reasons for this are complicated but often are related to epigenetic changes ...
iayork's user avatar
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2 votes
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Why is DNA polymerase added at the end of PCR reaction?

True, the template is double stranded, but the primers could begin amplifying themselves, and any other stray DNA. You don't want any nonspecific amplification in the early stages that could compete ...
Karl Kjer's user avatar
  • 7,693
2 votes
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Why does one combine PCR and cloning as ways for amplification of sequences?

There are several reasons to clone a PCR product but the main reasons include expressing the product and maintaining the library inexpensively. In the study you mentioned, the cloning and ...
WYSIWYG's user avatar
  • 35.6k

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