21
votes
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Can a human be made with ovum but without sperm?
Nice question! It is actually very difficult to do so because humans, obviously, are far more complex than some animal species which naturally show parthenogenesis. Just to mention, there are female-...
- 14.1k
20
votes
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Does a woman contain all the genes needed to make a man?
Human female cells contain most of the genetic information required to make a male, but they do not contain a critical component: The Y chromosome. This is a relatively small chromosome. Wikipedia ...
- 356
12
votes
Accepted
Are drones from one honey bee queen all clones?
The queen is diploid; the drones are haploid. Meaning the queen has two copies of each chromosome, and the drones have only one. So for each gene, the queen has two copies and passes one of those ...
- 3,245
12
votes
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What if target DNA doesn’t have restriction sites
If you're synthesizing the insert then you just design it with restriction sites that are compatible to the vector. Otherwise, you can design PCR primers that have restriction sites in them. That way ...
- 191
6
votes
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What are the problems in using an ssDNA or an mRNA for expression instead of using a plasmid?
What is the problem with handling pure mRNA nucleotides of gene X? Or
even sscDNA?
Problems in directly transfecting a mRNA/ssDNA
mRNAs can be transfected into the cells and is quite frequently ...
- 35.2k
6
votes
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Is it Theoretically Possible to Create a Male clone from the donor Cell of a Female?
So, i want to know if the sex of the resulting clone is determined by
the sex of the donor animal.
Generally yes for mammals and birds. In animals where the sex is determined by sex chromosomes, ...
- 2,916
6
votes
Plating 96-well bacterial transformations
I think your approach very much depends on how essential it is that you get discrete single colonies, and if this is a one off or a regular process. To preface, similar to what Maximilian Press said, ...
- 61
6
votes
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If we cloned an extinct animal, what would become of its gut biome?
The gut biome would be populated from whatever the environment was of the newly "cloned" animal.
Presumably the baby animal would be born by birth from a live host mother, which would be the ...
- 9,132
5
votes
Why we need an egg cell donor and a surrogate mother in an organism cloning process?
Could a whole set of artificial organism-cloning process only be
operated on a somatic nuclear donor?
No, this would not be possible. Single-cell reprogramming would enable you to reset the potency ...
- 134
5
votes
"Antibiotic resistance" equivalent in archaea for selection during cloning
Obviously, one can't use antibiotic resistance since they are not bacteria.
This is not so obvious. Some common antibiotics are active against archaea.1 The Halohandbook, an indispensable document ...
- 8,266
5
votes
Production of plant derivatives using genetic engineered micro-organisms
In principle, any biological product should be able to be developed through microbial synthesis, with the appropriate choice of chassis. Indeed, this was the goal of the DARPA "1000 Molecules&...
- 6,977
4
votes
Are progenies factually half-clones of the parents?
A progeny is not a half clone of its parents.
Each cell expresses both maternal and paternal genes. There are no parts of the body or even a cell for that matter that is identical to one of the ...
- 35.2k
4
votes
Accepted
Misuse of DNA leaks to create your clones, or children
As @iayork mentions in their comment, creating a human clone from a sequenced genome is still in the realm of science fiction. Also, DNA testing companies like MyHeritage don't sequence the client's ...
- 3,313
4
votes
Why is cDNA usually lacking in terminal sequences of the template mRNA?
Traditionally cDNA cloning used an oligo(dT) primer that binds to the 3′ poly(A) tail of a mRNA and extends towards the 5′ end of the mRNA.
What makes it difficult for the reverse transcriptase to ...
- 24.3k
3
votes
Are identical twins clones?
Are identical twins clones?
A clone is an individual that is genetically identical to another (see wikipedia > cloning). As you said, identical twins (better called monozygotic twins) are genetically ...
- 68k
3
votes
how do we prevent restriction enzyme of bacteria to cut and destroy our new recombinant?
You're essentially looking for a restriction modification system. You modify the restriction sites with methyl groups in a way that blocks restriction enzymes from accessing them. The DNA methylation ...
- 8,101
3
votes
In Sanger sequencing, why do we resort to cloning? Why doesn't PCR suffice?
The error rate of PCR is still very high in comparison to natural bacteria DNA replication of its plasmids.
Natural bacteria DNA replication has an error rate of approximately 1 in 10 billion, and ...
- 9,432
3
votes
Accepted
Genetic information storage and the extent to which it controls appearance/structure
The common phrase I've heard is "DNA isn't a blueprint, it's a recipe". More specifically, the nucleotides in DNA correspond to which proteins get made (coding DNA) and when and where (regulatory DNA)....
- 3,245
3
votes
Accepted
If a woman was fertilized with her own DNA, would it be her clone?
1)
In principle yes, you could in theory use micro injection to inject haploid DNA into the egg. I don't think this has been used in practice though. However Dolly the sheep was created by exchanging ...
- 679
3
votes
Isolating gene of interest from a large strand of DNA
Assuming that you know exactly where in the full DNA strand the gene of interest is located, it is fairly easy to isolate the gene. There are several restriction enzymes you can use, it's just a ...
- 511
3
votes
How can seedless fruits not be GMO?
GMO stands for Genetically Modified Organism.
Grafting a plant to a rootstock of another plant is a form of plant husbandry -- a farming technique -- but this isn't changing the DNA code of either ...
- 155
3
votes
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Alkaline Phosphatase and Ligase Protocol for Cloning
DNA ligase catalyzes the formation of a bond between 3’-OH and 5’-PO4 of its nucleic acid substrate. (See Lehnman, 1974) The ligation bond will be formed when the proper ends are close together, ...
- 556
3
votes
Accepted
Production of plant derivatives using genetic engineered micro-organisms
General Considerations
The question asks specifically why certain plant products are not produced commercially in genetically modified micro-organisms. There are some general reasons, illustrated in ...
- 24.3k
3
votes
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T-vector creation
Personally I would use Taq DNA polymerase to A overhang my inserts, especially if you are PCR amplifying the insert. It leaves an A overhang on the end of all amplicons natively. All you have to do ...
- 11.2k
3
votes
Using PCR to add the overhangs to gBlocks for NEB HiFi
You could add the overhangs with PCR, then gel purify or PCR purify. I don't think it will pose a big problem as I've done it before and all of the colonies I screened were correct.
- 31
3
votes
Hierarchial and shotgun sequencing vs Massively Parallel Sequencing
The construction of sequencing libraries for Illumina, PacBio, ONT, or similar instruments is a large field of methodology that encompasses a variety of different techniques. For background on ...
- 9,132
3
votes
Accepted
Why does plasmid cloning efficiency go down with increased size of insert?
I suspect, but don't have any proof, that it is the physical difficulty of getting both ends ligated to a single strand - think of it like this:
Your 10 kb is essentially a long string, ligate one end ...
- 11.2k
2
votes
Accepted
Freezing after restriction digest
From my experience I would say that it is no problem to freeze the digested DNA, when the enzyme is inactivated (even when not, for this time period I wouldn't expect much damage).
However, if ...
- 51.5k
2
votes
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In Sanger sequencing, why do we resort to cloning? Why doesn't PCR suffice?
You can use PCR products in Sanger sequencing; it is very common. Using PCR products instead of cloned genes does raise a set of problems that are less of a concern than with cloned sequences, such as ...
- 14.2k
2
votes
Accepted
Unwanted E. coli product in plasmid product
The root cause turned out to be a lying spec. Using a different one, it was revealed that there was next to no template DNA in the initial amplification. No DNA, no good plasmids.
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