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21 votes
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Can a human be made with ovum but without sperm?

Nice question! It is actually very difficult to do so because humans, obviously, are far more complex than some animal species which naturally show parthenogenesis. Just to mention, there are female-...
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20 votes
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Does a woman contain all the genes needed to make a man?

Human female cells contain most of the genetic information required to make a male, but they do not contain a critical component: The Y chromosome. This is a relatively small chromosome. Wikipedia ...
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13 votes
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Cloning and Telomeres

No, it doesn't seem to be common practice. A telomerase treatment would probably do more harm than good, and may be completely unnecessary in the first place. Betts and coworkers published a study in ...
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12 votes
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Are drones from one honey bee queen all clones?

The queen is diploid; the drones are haploid. Meaning the queen has two copies of each chromosome, and the drones have only one. So for each gene, the queen has two copies and passes one of those ...
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  • 3,197
12 votes
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What if target DNA doesn’t have restriction sites

If you're synthesizing the insert then you just design it with restriction sites that are compatible to the vector. Otherwise, you can design PCR primers that have restriction sites in them. That way ...
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10 votes
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How much DNA of Albert Einstein is recoverable?

During the generation of gametes (sperm, eggs), chromosomes can cross-over - this swaps paternal and maternally-derived genetic material. So none of the descendant's chromosomes would be a direct ...
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9 votes
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Does a man contain all the genes needed to make a woman?

This answer also involves some speculations as the question is about a good theoretical framework for a science fiction. You can find in this post about how sperm can be used to produce embryonic ...
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8 votes

Does a man contain all the genes needed to make a woman?

A woman (assuming no mosaicism) has two X chromosomes in the nuclei of her cells (except for oocytes). A man, in every cell with a nucleus (except for spermatocytes), has only one, pluripotent or not. ...
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7 votes
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Organ cloning - possible to make a non-antigenic organ?

I'll contradict the answer posted here (ages ago) and say no, it isn't possible. Growing an organ wthout MHC proteins might be achievable, but it would be rejected for not having MHC1. NK cells ...
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6 votes
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What is a suicide plasmid?

As can be inferred from the Shanks et al. paper linked in the question: In molecular biology, "suicide plasmid" is a term that refers to a plasmid which is replication incompetent.* Plasmids normally ...
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  • 4,460
6 votes
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Why are female clones more often produced

They aren't, anymore. It was a fair guess at the time, but first I think we should define what cloning in this context mean. Our own cloning tag says: The process in nature or in the lab by which ...
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  • 9,702
6 votes
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What are the problems in using an ssDNA or an mRNA for expression instead of using a plasmid?

What is the problem with handling pure mRNA nucleotides of gene X? Or even sscDNA? Problems in directly transfecting a mRNA/ssDNA mRNAs can be transfected into the cells and is quite frequently ...
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6 votes
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Is it Theoretically Possible to Create a Male clone from the donor Cell of a Female?

So, i want to know if the sex of the resulting clone is determined by the sex of the donor animal. Generally yes for mammals and birds. In animals where the sex is determined by sex chromosomes, ...
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  • 2,876
6 votes

Plating 96-well bacterial transformations

I think your approach very much depends on how essential it is that you get discrete single colonies, and if this is a one off or a regular process. To preface, similar to what Maximilian Press said, ...
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  • 61
5 votes

"Antibiotic resistance" equivalent in archaea for selection during cloning

Obviously, one can't use antibiotic resistance since they are not bacteria. This is not so obvious. Some common antibiotics are active against archaea.1 The Halohandbook, an indispensable document ...
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5 votes

Does a man contain all the genes needed to make a woman?

Theoretically this should be possible as men carry both sex chromosomes. You would have to find a way to make haploid cells and then have them form a diploid cell with two X chromosomes. Your ...
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  • 49.1k
5 votes

Why we need an egg cell donor and a surrogate mother in an organism cloning process?

Could a whole set of artificial organism-cloning process only be operated on a somatic nuclear donor? No, this would not be possible. Single-cell reprogramming would enable you to reset the potency ...
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  • 134
5 votes

Production of plant derivatives using genetic engineered micro-organisms

In principle, any biological product should be able to be developed through microbial synthesis, with the appropriate choice of chassis. Indeed, this was the goal of the DARPA "1000 Molecules&...
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4 votes

How bad is ethidium bromide in your plasmid for downstream applications?

Purification and isolation of DNA bands by cutting them from agarose gel is commonplace (Lee et al., 2012). The purification step after excision of the band gets rid of most of the EtBr and other ...
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  • 51k
4 votes

Is there a command line molecular cloning tool?

Making maps of plasmids Annotating plasmids with common features Plasmapper works through commandline Finding restriction sites Simple regex searches can do that Mapping Sanger sequencing ...
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  • 35k
4 votes

Are progenies factually half-clones of the parents?

A progeny is not a half clone of its parents. Each cell expresses both maternal and paternal genes. There are no parts of the body or even a cell for that matter that is identical to one of the ...
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  • 35k
4 votes
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Misuse of DNA leaks to create your clones, or children

As @iayork mentions in their comment, creating a human clone from a sequenced genome is still in the realm of science fiction. Also, DNA testing companies like MyHeritage don't sequence the client's ...
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3 votes

In Sanger sequencing, why do we resort to cloning? Why doesn't PCR suffice?

The error rate of PCR is still very high in comparison to natural bacteria DNA replication of its plasmids. Natural bacteria DNA replication has an error rate of approximately 1 in 10 billion, and ...
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3 votes
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Is the amount of dNTPs rate limiting for very long PCR products?

200uM dNTPs in 50ul reaction mix could produce more than 10ug DNA if all dNTPs were consumed whatever the length of PCR products is. You could detect as low as 5ng DNA fragments by conventional ...
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  • 1,116
3 votes
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What are the Gateway clonase enzymes?

The prototypes for these proteins are: the bacteriophage λ proteins Int and Xis, required for, respectively the integration and excision of prophages. They are, as mentioned by @WYSIWYG, ...
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3 votes

how do we prevent restriction enzyme of bacteria to cut and destroy our new recombinant?

You're essentially looking for a restriction modification system. You modify the restriction sites with methyl groups in a way that blocks restriction enzymes from accessing them. The DNA methylation ...
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  • 8,031
3 votes

Are identical twins clones?

Are identical twins clones? A clone is an individual that is genetically identical to another (see wikipedia > cloning). As you said, identical twins (better called monozygotic twins) are genetically ...
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3 votes
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Genetic information storage and the extent to which it controls appearance/structure

The common phrase I've heard is "DNA isn't a blueprint, it's a recipe". More specifically, the nucleotides in DNA correspond to which proteins get made (coding DNA) and when and where (regulatory DNA)....
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  • 3,197
3 votes
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If a woman was fertilized with her own DNA, would it be her clone?

1) In principle yes, you could in theory use micro injection to inject haploid DNA into the egg. I don't think this has been used in practice though. However Dolly the sheep was created by exchanging ...
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3 votes

Isolating gene of interest from a large strand of DNA

Assuming that you know exactly where in the full DNA strand the gene of interest is located, it is fairly easy to isolate the gene. There are several restriction enzymes you can use, it's just a ...
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