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1 vote

How to separate two plasmids from E coli with the same backbone?

There's a couple of options here - it could be that your plasmid extract isn't very pure, so some genomic DNA has come over and the ROI is in that. The PCR would detect this even if you don't have any ...
bob1's user avatar
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1 vote

ddT-tailed TA cloning, and the fate of a double nicked plasmid in E. coli

To answer question (4): A-tailing can be done in multiple ways, including the way you described. See here for the most common protocols:
Felix H.'s user avatar
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