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Personally I would use Taq DNA polymerase to A overhang my inserts, especially if you are PCR amplifying the insert. It leaves an A overhang on the end of all amplicons natively. All you have to do here is add some Taq at the end of the reaction and incubate for 15 min at 72 $^\circ$C, it'll work in pretty much any PCR buffer and many restriction enzyme ...


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General Considerations The question asks specifically why certain plant products are not produced commercially in genetically modified micro-organisms. There are some general reasons, illustrated in some of the examples mentioned: The product is actually a complex mixture, rather than a single compound. This is the case for saffron, preparations of which ...


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