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It would make the result at least a little ambiguous. How would you establish with high confidence that the offspring was the results of your implanted cloned ovum and not a naturally fertilized one? DNA testing would have to be quite extensive to establish that offspring was a clone and not the result of an ordinary pregnancy. If you use a surrogate mother, ...


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There are 3 versions (a,b, and c) of this plasmid with different multiple cloning sites. You can safely ignore the "(+)" part unless you are packing your plasmids into viruses. You can learn about the details and see sequences from the resources available on the Millipore website. I found this by searching for "pET-28 a(+)" ...


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