5 votes
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Detecting food fraud

There are several ways you could go about identifying species through DNA. If you want to do everything yourself, the simplest option in terms of equipment needed consists of evaluating fragment ...
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  • 3,488
4 votes
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Mold identification

I initially figured it was condensation from the agar. But it is not on your lid and you said you suspected humidity was low. I found this here http://www.gettyimages.ca/detail/photo/mold-...
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4 votes
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Beginner question about growing E. coli

The higher the temperature the faster E. coli will grow, with a maximum growth rate around 37 Celsius. If you grow E coli at lower temperature, the ecoli will grow slower. At 37°C you will see ...
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3 votes

DIYbio - CRISPR injection sites for targeting the ABCC11 gene

Creating changes in the genome in order to get your favorable results is not always as easy as it looks. Expression of a particular gene is not necessarily bound to its existence. There are other ...
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3 votes
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Why electroporator need a cuvette?

Generally I would advise against making a home-brew electroporator: the voltage needed for electroporation is so high, that even with professional equipment you can easily get arc lightning. In an ...
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  • 4,296
2 votes

How to obtain a tardigrade?

Tardigrades can be found in almost every habitat! The easiest way to find them is probably to look in lichens or mosses (although it depends where you live). serc.careton.edu website suggests a ...
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2 votes

How to supply oxygen to a culture vessel?

Gases are supplied to culture vessels by design. Flasks, for example, may have filtered caps that allow gas in, or microplates with lids that have enough room under the lid to allow a gas exchange ...
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1 vote

Reliable recipe to make DIY yeast slants

Yeast grows very well in liquid. The initial innoculum from the store will visible grow (“prove”) in warm water with a little sugar.
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1 vote

Are there COVID-19 citizen science projects to compare habits and outcomes?

I think you are looking for a very specific design of 'citizen' projects. But, if we relax a bit your criteria, there are some examples. Here, and here, for instance. One worth discussing, I think, is ...
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1 vote

DIY extraction and long term storage of human DNA sample?

If you have access to a centrifuge, either spin columns or phenol:chloroform will give very pure, high-quality DNA. Even with dirty methods, you can re-extract the product several times, to sacrifice ...
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  • 4,470
1 vote

Real Time PCR Test Chemistry

There are two different ways to detect PCR product in real-time PCR: dyes which bind to DNA in general, such as SYBR Green I, or probes specific to your PCR product. There's a summary of the benefits ...
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