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2 votes

What evidence is there that common PCR DNA purification kits can isolate DNA from pathogens engulfed in macrophages?

For this problem, I would generate the evidence myself taking the following approach: Apply the DNA extraction kit to the macrophages with pathogens engulfed. As controls, also use the DNA extraction ...
3 votes

Origin of the 260/280 ratio?

Here's one explanation: It's not the base composition that determines UV absorbance (260 nm), but whether the nucleic acid is in a single- or double stranded state. Promega cites Griffiths biochemical ...
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