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are the primers used in every cycle the same sequence? Yes. You put the whole reaction on the thermocycler, and then walk away until all the cycles are complete. But one could do a second round of PCR with different internal primers on that first PCR product, if you wanted. Second, why are primers not reusable for different cycles? Are you asking why you ...


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The primer is incorporated into the start of the new strand. The number of dna strands produced is limited by the number of primers.


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