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This graph from the Broad's Opinionome blog (ugh) is somewhat more annotated: As noted elsewhere, the precipitous drop in 2007 is almost certainly due to maturing next-gen sequencing (NGS), in particular Illumina. Illumina acquired Solexa in 2007, which offered gigabase-level sequencing ability. In 2014, President of Illumina Francis de Souza essentially ...


I'm not 100% sure, but I think Illumina launched their HiSeq machine around that time, massively increasing the sequencing output in one run. That dip might be related to that.


The graph is in contradiction with the data in the Excel spreadsheet the same webpage provides ( (mirror)), which I plotted below:\$1,000_genome&oldid=902062326#Commercial_efforts to some of the dates on the ...


Some specific artefacts are: apparent insertions or deletions in the sequence, which are really just skipped base or duplicated base errors during the sequencing apparent variants which do not exist in the sample but are introduced in the data because of miscalling at individual bases differences in abundance of variants between the output and the sample ...


Artifacts are variations introduced by non-biological processes. Sequencing involves a lot of chemical reactions, and changes in nucleotides that can seem like mutations are really introduced somewhere in the processing of the sample. That's what we call "sequencing artifacts". Proper experiment design, with careful replication, should help you clarify which ...


I believe an artifact is something that appears in the first analysis but is not actually present in the sample. You would want to sort out all of the artifacts so that your final analysis is accurate.

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