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3% and 99.9% are from different calculation method. 20% (a rough estimation, might be wrong) of neanderthal DNA survived in the modern human genome, but it is 'diluted' by the human population, which lead to 2 percent of neanderthal DNA in each human genome. This is the number 3%. But how can we know it's neanderthal DNA? If DNA fragment contains ...


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PCR results in double stranded DNA. During Sanger sequencing, single primers are used to amplify each of the two strands separately, so that one strand can confirm the other.


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