4 votes
Accepted

Is it possible to proccess human genome in a local computer?

I don't know what you mean by "process". Aligning to the human genome might be a strain on your computer, since it has to hold the whole index in memory, but I don't think you'll have to do ...
swbarnes2's user avatar
  • 5,230
4 votes
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How do we know that the DNA we share with other species (especially primates) isn't fully the result of transposable elements?

Never say never in biology. But it is not plausible that TEs explain the similarity in great apes (including humans). Much (most?) of the argument for similarity is and has always been due to ...
Maximilian Press's user avatar
4 votes

Can a deletion occur on only one strand of a human chromosome?

As it appears that most deletions occur by mechanisms that do not involve large-scale strand separation (e.g. during crossing over or by translocation etc.) this is unlikely to happen (see e.g. ...
David's user avatar
  • 25.7k
4 votes

Can a deletion occur on only one strand of a human chromosome?

This is a somewhat semantic question. In terms of WHEN a deletion happens, I think it's commonly on only one strand as most mutations are caused by a collision from radiation or sometimes a chemical. ...
shigeta's user avatar
  • 28.2k
4 votes
Accepted

Change of DNA concentration due to restriction digest?

Summary The poster wishes to know why spectrophotometric readings (presumably absorbance at 260 nm) on a sample of genomic DNA from an unspecified organism appear “slightly” greater after incubation ...
David's user avatar
  • 25.7k
3 votes
Accepted

Preserving a sample for mtDNA and nuclear DNA analysis

If you're wanting to store DNA long-term at room temperature, you'll probably want to dry it. DNA extraction methods have been known since 1869. You can then use ethanol to precipitate the DNA, ...
Darlingtonia's user avatar
  • 2,552
3 votes

Change of DNA concentration due to restriction digest?

As you did a clean up, then you lost some DNA in this process. There are always losses from clean up steps. Note also that spectrophotometry is notoriously unreliable for DNA quantitation; it's more ...
bob1's user avatar
  • 12k
3 votes
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Are DRB3 DRB4 and DRB5 different genes or different version of the same gene

The reference to DRB3, DRB4, and DRB5 as paralogues of DRB1 indicates that these are different genes. However, as paralogues they share essentially the same structure, having presumably originated ...
Darlingtonia's user avatar
  • 2,552
3 votes

what are average, median, and minimal dna closeness of stranger peoples, compared to closeness of relatives?

0.1% and 0. the average person varies by around 0.1% from another random person, or two random people are 99.9% the same. Humans actually have very low genetic diversity as a species. Total variation ...
John's user avatar
  • 14.7k
3 votes

Telomerase and End Replication in Eukaryote

The overhangs are needed for telomere function and maintaining chromosome stability, so they aren't deleted. This is an active area of research, so the amount of detail you need to know depends on ...
BigMistake's user avatar
2 votes

Where can I find the genome of the L-strain coronavirus and the S-strain coronavirus?

I know it's been a while, but in case anyone comes back to this: since the beginning of the pandemic, many tools have been developed to help identifying lineages and clades. L-Strain corresponds to ...
Cornelius Roemer's user avatar
2 votes
Accepted

Can a dividing cell that skipped DNA replication become cancerous?

Question 1 Will the Spindle Checkpoint fail due to not having duplicated DNA, or can mitosis complete and form aneuploid daughter cells? Yes it is possible. Meiosis 2 essentially does not involve ...
WYSIWYG's user avatar
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2 votes
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How to quantify and measure phenotypic variation?

It depends on the phenotype you're quantifying. If you want to quantify height, for example, just measure it and record the number. However, you seem to be implying "overall" phenotypic ...
BigMistake's user avatar
2 votes
Accepted

If sufficienly many people are born, then is it possible that two persons are genetically identical although they are not twins?

At a very crude level, the full human genome contains 6 billion nucleotide pairs. Each of those nucleotide pairs has four possible values: AT, GC, TA, and CG. That means there are $4^{6e9}$ (4^(6 X 10^...
Charles E. Grant's user avatar
2 votes
Accepted

What do alleles look like visually on linear DNA strands?

I think I may understand the point of difficulty the poster is encountering, and have produced the diagram below (adapted from an internet source) in the hope that it may help. This diagram shows the ...
David's user avatar
  • 25.7k
2 votes
Accepted

Reverse protein biosynthesis in nature

In biological systems, the central dogma states that genetic information flows from DNA to RNA to proteins, and the reverse process, synthesizing DNA or RNA from proteins directly within cells, does ...
Chris's user avatar
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2 votes
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How do molecular biologists typically get their oligos?

Depends. For short defined sequences, IDT or a similar oligo synthesis provider is your best bet. It's very fast and straightforward, compared to trying to do something complicated with a genome. ...
Maximilian Press's user avatar
2 votes

How many generations does it take for the average descendant not to be genetically related to the ancestor?

Graham Coop performed simulations related to this question, based on actual transmission data for each chromosome in humans. He found that after 10 or 11 generations there was >50% chance that any ...
Darlingtonia's user avatar
  • 2,552
2 votes
Accepted

qPCR precipitation

I would say, in general the answer is yes, with caveats. Firstly, remember that the optimal amplicon size for qPCR is small, so you can expect lower yields. Secondly, what is the primer concentration ...
Andrew Roots's user avatar
2 votes

Is it possible to plant tardigrade DNA into a human? What would happen even if?

Yes, parts of tardigrade DNA that code for specific proteins or abilities could potentially be isolated and inserted into human cells in vitro. Getting them into a living human is technically possible,...
BigMistake's user avatar
1 vote

Can DNA reveal an approximate chronological generation of a person?

Briefly, no. There may be an argument for using similarity to previously observed archaic hominin genomes that you could impute the age by similarity to e.g. Neanderthals. But those would be very old ...
Maximilian Press's user avatar
1 vote

Preserving a sample for mtDNA and nuclear DNA analysis

For anyone curious, @Darlingtonia's answer is what I needed. It also gave me the search terms that Google could use to finally find further useful information. In particular, a paper published at ...
CXJ's user avatar
  • 133
1 vote

Can a person have different sex at cellular level?

Every organism(sexually reproducing) starts life from a single cell zygote formed by union of sperm and ovum. Since all cells are formed by mitotic divisions and differentiation of the zygote, the ...
Aurelius's user avatar
  • 197
1 vote

How many base codes are in DNA? Two or four?

This does not appear to be a question about biology but a semantic question in the realm of information technology. However, the answer is NO. First, linguistically I would never use the word “coded” ...
David's user avatar
  • 25.7k
1 vote

How many base codes are in DNA? Two or four?

Can we say that the DNA is coded binary with two options: AT and GC? Sort of, but really in one aspect. DNA is double stranded. It is replicated by splitting the strands apart and generating two new ...
Charles E. Grant's user avatar
1 vote

How many generations does it take for the average descendant not to be genetically related to the ancestor?

Father/Mother, DNA = D Child, $\frac{1}{2} \times D$ Grandchild, $\frac{1}{2}\times \frac{1}{2} \times D= \left(\frac{1}{2}\right)^2 \times D$ Great grandchild, $\left(\frac{1}{2}\right)^3 \times D$ ...
Agent Smith's user avatar
1 vote

Inheritance of child behavior based on daily life experiences of the parent

Children inherit DNA from their parents, which in turn was inherited by their parents from their grandparents, and so on. Only modifications to the DNA in the germ-line cells (those producing eggs and ...
Bryan Krause's user avatar
  • 45.7k
1 vote

Calculating pitch of B-DNA

The answer is mostly that you have to use the right denominator, as suggested by @DrRadium's comment. The crucial mistake in the other answer was measuring 9bp and dividing by 10. That's measuring the ...
Maximilian Press's user avatar
1 vote
Accepted

ddT-tailed TA cloning, and the fate of a double nicked plasmid in E. coli

To answer question (4): A-tailing can be done in multiple ways, including the way you described. See here for the most common protocols: https://international.neb.com/applications/cloning-and-...
Felix H.'s user avatar
  • 318
1 vote

What makes protein binding to the DNA random?

Crossovers during Meiosis are not random in human beings. Its an interesting question as to whether there are organisms that are completely random but the reasons we will discuss here, that is pretty ...
shigeta's user avatar
  • 28.2k

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