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Why does plasmid cloning efficiency go down with increased size of insert?

I suspect, but don't have any proof, that it is the physical difficulty of getting both ends ligated to a single strand - think of it like this: Your 10 kb is essentially a long string, ligate one end ...
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Do complementary DNA sequences/codons produce the same amino acids?

Transcription is a highly regulated process, cells aren't just producing random RNAs from random stretches of DNA. The transcription machinery needs to bind to DNA to get started, which requires a ...
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Non fluorescent DNA stain to visualize Drosophila polytene chromosomes

Giemsa stain should work. It is traditionally used in karyotyping staining. It results in G-banding.
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Plasmid Design and Integration events [Single vs Double cross over]

Single crossover is not the result of religation in the cell. The double-stranded break (DSB) in the plasmid instead stimulates a homologous recombination event, causing the entire plasmid to ...
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What results would come from DNA analysis of several different types of DNA mixed together?

You may be interested in commercially available meat testing kits - DNA kits have been widely used to detect what certain food products are actually (or are actually not) made of. Some kits are ...
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