5
votes
Accepted
How was Campbell's Model of phage lambda integration into E. coli definitively proven?
The Landy and Ross (reference 15)1 article in the review leads is an article freely available on PubMed Central.
Within in this review, there are further references, including a further review by ...
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5
votes
Accepted
Did early 20th century researchers state why they used E. coli as a model organism?
Historical Fallacies implicit in the Question
“…why a researcher chose to use E. coli as a model organism.”
Researchers did not work with E. coli because they regarded it as a “model ...
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4
votes
Recombination frequency as a function of genomic location in E coli?
A recombination map of the E. coli genome was recently published (several years after question was asked).
- 10.5k
4
votes
Did early 20th century researchers state why they used E. coli as a model organism?
Very interesting question.
Did early 20th century researchers state why they used E. coli as a model organism?
In short: No (at least in the 1920ies). For instance: Werkman 1927: Vitamin Effects ...
- 1,597
4
votes
Accepted
Why do coliform bacteria smell so bad?
E. coli and other bacteria metabolize tryptophan into odoriferous skatole and other indole compounds.
If you're culturing these organisms in medium that contains tryptophan, that may be what you're ...
- 5,590
3
votes
I accidentally plated DH5a E. coli and left them in the 4℃ for 12 hours, if I put them into the 37℃ incubator will they start growing?
If these were just regular E. coli that you were growing up, then it wouldn't matter too much - they would certainly grow a lot slower on the plate than you might otherwise expect compared to a plate ...
- 12.9k
3
votes
Mutations in a Petri dish overnight
We can calculate the number of base pair copy errors in a Petri dish per hour by multiplying the number of base pairs (B), the copy error rate (P), the number of divisions in an hour (D), and the ...
- 539
3
votes
Why do E.coli form parallel clusters?
Cell division happens by division at the middle of the rod, so the result is two daughter cells that are at nearly the same angle. Over time in the absence of agitation moving cells around, this will ...
- 10.5k
3
votes
Plotting metabolic networks?
This is an old question, but Escher is a web app that has been developed that now enables the drawing of these maps using a web tool. Further customization (like reaction colors) could be manually ...
- 31
2
votes
Accepted
Why do two different E. coli reference genomes have different lengths?
U00096.2 is an updated version of U00096.1; you should preferably use the former for your analysis. In fact, even U00096.2 has been updated. The latest version is U00096.3. In general, the number ...
- 35.6k
2
votes
Accepted
Are there ways to genetically increase mutation rate of E.coli?
Mutations are caused by the insertion of the wrong bases during replication, or by chemical changes to bases, either by chemical agents or by radiation.
The rate of mutations could be increased by ...
- 1,473
2
votes
Why pellet and resuspend E. coli for plasmid prep
The reason this centrifugation/resuspension step is done has a simple reason: It concentrates the bacteria in a much smaller volume which is much easier to handle afterwards.
For a maxiprep you ...
- 52.5k
1
vote
Accepted
Correlation of Meselson and Stahl with “multifork” replication in E.coli
It is sometimes considered that the result was an artifact for a reason similar to that which you mention. However, it happened to be correct in its conceptual outcome, and was not rejected by ...
- 10.5k
1
vote
Why do E.coli form parallel clusters?
Bacterial cells prefer to attach with each other in parallel orientation so that they have maximum surface of contact and could form biofilm clusters.
Both physical factors (like Brownian motion, ...
- 1,317
1
vote
How to estimate mRNA counts from Relative Promoter Units (RPU) or RNAP per second (PoPS) in E. coli?
I could not find any paper that discusses these issues. So this answer is just based on my guess.
From the this page:
Transcription rate is generally a parameter associated with a
particular ...
- 35.6k
1
vote
How can I improve efficiency of Ecoli transformation?
The plasmid is quite big which might explain why it is difficult to transform bacteria (is it a lentiviral vector?).
If you could give additional details you might have a chance to have improved ...
- 620
1
vote
E. Coli metabolization of paracetamol
Sorry that my answer is not a real answer, but this would be a good starting point for some more science.
Several of the questions you might ask and could answer (with some more experiments):
Does ...
- 1,473
1
vote
Dissolving cell pellet after sonication
Follow the instructions that came with your column for cleaning. The resin will probably be fine, but you'll need to thoroughly clean the column. My GE columns recommend washes of:
20% EtOH
30% ...
- 1,301
1
vote
Plasmid specific DNA degradation
traditional method to remove a plasmid from E. Coli is to grow the cells with Etidium bromide.
http://vlab.amrita.edu/?sub=3&brch=186&sim=1097&cnt=1
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Why do two different E. coli reference genomes have different lengths?
As WYSIWYG answered, they are updated versions.
The difference in length is mainly because that later versions are sequenced / aligned with better equipement and techniques, providing results that ...
- 868
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vote
Whence fecal E. coli (et al.) if swallowing it is dangerous?
There are many varieties of E. coli, with differences in their chromosomal DNA, different plasmids (independent loops of DNA that replicate autonomously inside the host cell and can be transferred ...
- 747
1
vote
Accepted
What nutrients are best suited for growing E.Coli
Optimal is a funny thing; it depends upon what you want. The purpose of the bacteria, is probably the most important aspect when considering the nutrients. There are many recipes capable of growing E. ...
- 375
1
vote
How does a bacterium age without cell division?
First of all, the growth of a bacterium is associated with an increase in cell biomass.So if a bacterium is restricted from replicating it does not grow. Coming to your later parts of the questions ...
- 11
1
vote
Codon tables and the wobble hypothesis
This is a good question as, although in higher eukaroyotes codon usage does not necessarily correlate with translational efficiency, it does to a large extent in Escherichia coli (see e.g. Boël et al.,...
- 26.3k
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