4
votes
Sensitivity vs. Limit of Detection of rapid antigen tests
Not necessarily. Sensitivity and specificity are decision criterion-based measures. They are not actually separable from another, they are a consequence of choosing a threshold at which to say "...
- 41.2k
3
votes
What is the advantage of indirect ELISA over direct one?
You are correct, the selectivity advantage of an indirect or sandwich ELISA comes from the fact that two antibodies are employed - one to capture the analyte, the other to detect it.
Here is the ...
- 15.2k
3
votes
Accepted
About sandwich ELISA
This page from Cole-Parmer includes everything you could possibly want to know about the adsorption of biomolecules to polystyrene. To summarize:
binding is neither covalent nor ionic, but
it is ...
- 15.2k
3
votes
Can ELISA be used to detect a plant enzyme? Creating assay for a new enzyme
I want to add something on top of Chris answer.
The production of an antibody it is usually a quite slow (and expensive) process, an alternative that worth to consider is phage display (https://en....
- 3,068
2
votes
What could cause individual false positives in ELISAs, while the rest of the plate looks fine?
Is your detection or secondary antibody aggregating? I've done extensive clinical ELISA development in the past and when this problem inevitably crops up for these assays the most likely culprits in ...
- 226
2
votes
TMB vs ECL for ELISA: which detection method is more sensitive?
In general, ECL is more sensitive than TMB for ELISA detection. The minimum detection limit of TMB is 60pg/mL and 20pg/mL for ultra TMB [1], whereas the detection limit of ECL can be as low as about 1....
- 418
2
votes
Why can't we always create a vaccine against a virus when an ELISA test to detect it is possible?
I found the question quite interesting and will to provide a concept and a speculation although I could not answer the question to my own satisfaction (edits to this regard are welcome)
Currently (...
2
votes
Can ELISA be used to detect a plant enzyme? Creating assay for a new enzyme
I think the most promising routes use antibodies. You could either develop an ELISA or do western blot analysis of plant material - both need a good and specific antibody. To generate these, the ...
- 51.2k
2
votes
Rationale behind washing off unbound protein antigen in indirect ELISA
The method is based on the principle that the amount of antigen bound to the plastic will be proportional to the concentration in the sample being tested. And of course an ELISA will usually be run ...
- 22.7k
2
votes
What is the advantage of indirect ELISA over direct one?
It's often not necessarily an advantages vs disadvantages question, but one determined by available reagents and what question is being asked.
For example, if you are developing a test for the ...
- 711
1
vote
Background correction when reading ELISA with TMB substrate
Using different wavelength for reference give some advantages. You can measure a baseline of each well. All well might not have the same baseline because each well might not be the exactly same as ...
- 1,116
1
vote
What is the advantage of indirect ELISA over direct one?
I agree with the other answers and the biggest difference is indeed the specificity. An indirect ELISA is indeed more specific, but also for a reason which isn't described here yet: Using indirect ...
- 92
1
vote
Why there is no ELISA for SARS-CoV-2?
once you are comparing ELISA with RT-PCR i will assume we are speaking about antigen tests for SARS-CoV-2. In that sense, we can note that whichever type of ELISA chosen, all of them will depend on ...
- 198
1
vote
Why there is no ELISA for SARS-CoV-2?
I agree with @mpprogram6771 and @iayork. Development of ELISA kits consumes more time to develop and validate - the first on the market last year were not recommendable.
Now a variety of immunoglobuin ...
- 11
1
vote
ELISA troubleshooting
The full oxidation of TMB ( 3,5,3’,5’-tetramethylbenzidine) is 2-electron process. The first step (one electron oxidation) gives the blue product, which is then further oxidized to a yellow product (...
- 49
1
vote
Accepted
Can experiments in ELISA kits be monitored via both fluorometry and photometry?
can we use both methods depending on whether the marker enzyme on the antibody is given a fluorescent or chromogenic substrate?
This is essentially correct.
You can have the same ELISA work on ...
- 8,091
1
vote
Calculating CV for intra-assay ELISA
As far as I am concerned the intra-assay CV is the average of individual CVs found in an assay.
So let's assume you have a data set of 40 samples, and for each sample you have 2 replicates (results), ...
- 1,509
1
vote
the range of interleukin 2 and 12 that can be used to activate nk-92 cells
If you have the resources you should ideally titrate your cytokines. You'll find that cytokines from different vendors may exhibit varying stability or availability in vitro, but also that you obtain ...
- 8,091
1
vote
Concentration calculation of Factor VIII for Elisa
My answer: 4.79 µg/ml
(All values below are µg/ml)
Calibration data
Determine the mean of the four absorbance values at each dilution.
The calibration curve is then a plot of concentration (x-axis)...
- 22.7k
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