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Within reason, you can basically use any buffer you like with Sephacryl S-100, provided that is suits your purpose in terms of protein/enzyme stability, and phosphate-buffered saline sounds ideal. There are sometimes a small amount of positively or negatively charged groups associated with a gel-filtration resin, introduced during the manufacturing process, ...


2

Per your prompt, there are three new brands of fish food, but... You have been using a standard type of food that has worked reasonably well. I suggest you include the "standard" fish food as a control, so that you will have four treatments in all. Besides that, the experimental framework suggested by Willk is spot-on.


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This is a good experiment! 3 types of food: ok 9 tanks: ok Apply different treatment randomly - no! You are right - no way to tell with random. It is like comparing McDonalds to vegan diet - you have to stick with your diet to be able to tell a difference in the end. So Tank 1,2 and 3 get food A. Tank 4,5,6 get food B. Tank 7,8,9 get food C. Each tank ...


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The phenomenon you are referring to is called microdosing. It was brand-new in terms of research focus when you initially asked your question, and it is still a fairly novel and little-studied subject. A lot of interest in this phenomenon stems from anecdotal evidence by microdosing users, who report beneficial effects. This is however not necessarily an ...


1

A more useful question to ask would be, Why do you place the disc on the plate before the bacteria have grown up? This test allows us to infer which antibiotics an organism is susceptible and resistant to by observing whether or not they grow in the presence of that given antibiotic. But there's a good reason we don't wait until the bacteria are already ...


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Personal anecdote: Once I planned to sterilize seeds of Silene latifolia for tissue culture but after the first step (washing the seeds in water for an hour) I had to do other things and the seeds did spend the night wet in room temperature. The next day some of the seeds were showing first signs of germination - a little crack in seed coat. I decided to ...


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Hi again TheLast Cipher, Enzymes in a cell are usually semi-quantified by the: Western Blot technique. But there are lesser known techniques. E.g. For a Western Blot the unit of measurement is the color intensity of the enzyme band on the blot membrane. If I were you I would look for journal articles covering your pathway of interest. E.g. https://iubmb....


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Theoretically, if refractive index and viscosity are both simply functions of moisture content, you should be able to use one to approximate the other using an empirical standard curve. However, based on this paper, it looks like the relationship between moisture content and viscosity at 25C can vary quite a bit between different types of honey, expecially ...


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