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Per your prompt, there are three new brands of fish food, but... You have been using a standard type of food that has worked reasonably well. I suggest you include the "standard" fish food as a control, so that you will have four treatments in all. Besides that, the experimental framework suggested by Willk is spot-on.


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This is a good experiment! 3 types of food: ok 9 tanks: ok Apply different treatment randomly - no! You are right - no way to tell with random. It is like comparing McDonalds to vegan diet - you have to stick with your diet to be able to tell a difference in the end. So Tank 1,2 and 3 get food A. Tank 4,5,6 get food B. Tank 7,8,9 get food C. Each tank ...


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The phenomenon you are referring to is called microdosing. It was brand-new in terms of research focus when you initially asked your question, and it is still a fairly novel and little-studied subject. A lot of interest in this phenomenon stems from anecdotal evidence by microdosing users, who report beneficial effects. This is however not necessarily an ...


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Hi again TheLast Cipher, Enzymes in a cell are usually semi-quantified by the: Western Blot technique. But there are lesser known techniques. E.g. For a Western Blot the unit of measurement is the color intensity of the enzyme band on the blot membrane. If I were you I would look for journal articles covering your pathway of interest. E.g. https://iubmb....


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Theoretically, if refractive index and viscosity are both simply functions of moisture content, you should be able to use one to approximate the other using an empirical standard curve. However, based on this paper, it looks like the relationship between moisture content and viscosity at 25C can vary quite a bit between different types of honey, expecially ...


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Flow cytometry looking for your plasmid. These folks labeled their plasmid with a proprietary fluorescent marker, then used flow cytometry to detect cells which had taken up the plasmid. A novel rapid and reproducible flow cytometric method for optimization of transfection efficiency in cells An alternative and more direct method to using fluorescent ...


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You will get a much more complete answer on a statistics forum, or by simply consulting some basic tutorials on linear regression. But a little intuition should tell you that setting a cutoff on the value of the slope does not make sense. For instance, suppose you measured heart rate in beats per second instead of beats per minute. All the y-values would be ...


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You could try methyl cellulose, which my lab and others use. It's not exactly an adhesive, but it is quite viscous. It's common enough that it's in the Zebrafish Book: https://wiki.zfin.org/display/prot/Methyl+Cellulose+Mounting . Obviously, be sure whatever you do is approved by your animal care and use committee.


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