5

I have pondered life on Venus. I think the biggest problem would be lack of free water. The atmosphere is thick and under high pressure, and polar CO2 and H2SO4 like to bind with water. There is no water there. Mass action would pull water out of any concentration of water (like a Nostoc blob) and into the waterless density of the adjacent atmosphere. ...


4

Okay, you have a lot of questions there. I'll try to give some answers, but if you want more details or background knowledge I'd recommend you to read /study more about immunology (none of your questions are actually about molecular biology or cell biology). 2) Foreign cells are generally recognised by the immune system, based on the HLA molecules on the ...


1

I am not sure about what they used in 1958 (perhaps Sorvall). However you may look at recent papers for ECM protein isolation (if that was your objective). Have a look at this article. From materials and methods: ECM protein isolation. Fibroblasts or A431 cells were removed from the surface of the culture plate with 2 mM EDTA in PBS for 3–5 min at 37⁰C. ...


1

Generally the coating is of poly D-Lysine. Proteases act only on L-amino acids. So, I guess nothing should happen to the usual coated flasks. I have reused the flask even after 2 rounds of trypsinization, and the cells seemed to be fine. In your case, the matrix is made of proteins isolated from animal sources. They are susceptible to proteolysis. You are ...


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