7 votes

Is it possible to detect whether an organism has been gene edited (by humans)?

If you had access to the DNA of the organism's parent(s) then you could make an inference about whether the child's genome is consistent with being edited, assuming that the edit was done early enough ...
Jay Moore's user avatar
  • 827
5 votes

Can several genes be edited at once and what is the name of that procedure?

One of the advantages of CRISPR/Cas9 is that it allows what is called multiplexing. In other words: transcriptional regulation of multiple different genomic loci in one single construct. I'm not sure ...
pawelek zzz's user avatar
  • 1,087
3 votes

Can several genes be edited at once and what is the name of that procedure?

What you should do is highly dependent on what you are looking at and why you want to make those changes. It also depends on which method you want to use There are quite a few different ways of doing ...
bob1's user avatar
  • 12k
3 votes
Accepted

Limits of gene editing

Your question is very broad, but I'll try to address each of your points briefly. It would be nearly impossible to edit the genes in every cell of a human being or other complex organism simply due ...
MattDMo's user avatar
  • 15.3k
3 votes

Gene knockdown vs gene knockout vs knocksideways?

Hello and welcome Adil Amchi. In genetics and molecular biology there are differences between the terms knock-out, knock-down and (the lesser known term) knock-sideways. Knock-sideways: Inactivates ...
Andrew's user avatar
  • 714
3 votes
Accepted

Is it possible to reduce maturity time of plants with biotechnology?

Perhaps investigate the families of YUC genes that synthesize the growth hormone auxin (ncbi.nlm.nih.gov/pmc/articles/PMC6941117) and ARF genes (ncbi.nlm.nih.gov/pmc/articles/PMC4737911) that respond ...
Alex Reynolds's user avatar
2 votes
Accepted

DNA described somewhere?

It might be useful to look at how 23andme works, to see how this analysis product is generated, in general: The 23andMe genotyping platform detects single nucleotide polymorphisms (SNPs). A SNP is ...
Alex Reynolds's user avatar
2 votes

When does the Cas9 nuclease stop?

Regarding the loss of Cas9 activity, you are already touching on the answer in your question. Cas9 as a nuclease/enzyme is always active, and will continue to cleave double-stranded DNA as long as ...
gaspanic's user avatar
  • 628
2 votes
Accepted

Can the activity of a specific gene in a cell artficially be increased?

There are technologies available for inducing higher (or lower) gene expression in cultured mammalian/human cells as well as in bacteria. The state of the art is CRISPR-mediated gene activation/...
vopinator's user avatar
1 vote

Is it possible to reduce maturity time of plants with biotechnology?

Independent of the hormone manipulation suggested by Alex Reynolds, perturbation of transcription factor UPB1 has been found to have an effect on plant growth by Tsukagoshi and colleagues -- ...
acvill's user avatar
  • 8,296
1 vote

Can the activity of a specific gene in a cell artficially be increased?

A sequence is executed with transcriptase. To double amount of signaling or some protein production try doubling the sequence occurrence.
Serg Kryvonos's user avatar
1 vote

Can the activity of a specific gene in a cell artficially be increased?

If you are after large amounts of a particular protein, search "recombinant protein production". It is a huge field.
Polypipe Wrangler's user avatar
1 vote
Accepted

How to design a permanent vaccine to cholera using CRISPR?

So, would it be possible to engineer a CRISPR gene editing tool to prevent Vibrio Cholerae from getting into our cells? Vibrio Cholerae doesn't get into our cells. Did you observe that your paper ...
swbarnes2's user avatar
  • 5,230
1 vote

DNA described somewhere?

This question is both easy and hard to answer: DNA and its (known) functions have been described in scientific literature, textbooks and databases for the last few decades, but because this is a ...
Nicolai's user avatar
  • 4,391

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