Yes, Agrobacterium is indeed a very widely used vector in plants. So it wouldn't be wrong to consider bacteria as vectors. Just to add, it's worth noting that in recent times, bacterial vector options have been explored in the case of humans also, especially in the case of gene therapy for cancer treatment, though its success hasn't been demonstrated yet. ...


There are technologies available for inducing higher (or lower) gene expression in cultured mammalian/human cells as well as in bacteria. The state of the art is CRISPR-mediated gene activation/repression, where you fuse a transcription activation domain to a Cas gene in a CRISPR-Cas system (usually Cas9), and provide a guide RNA to target genes of interest. ...


A sequence is executed with transcriptase. To double amount of signaling or some protein production try doubling the sequence occurrence.


If you are after large amounts of a particular protein, search "recombinant protein production". It is a huge field.


Agrobacterium tumefaciens and Agrobacterium rhizogenes are soil-based plant pathogenic bacterial strains containing plasmid. This plasmid is known as Ti plasmid and is responsible for inducing tumor. Part of this plasmid called T-DNA can be integrated into the host chromosomes. So, this bacterium plasmid act as vector, but not the whole bacterial cell. (...

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