Really interesting question: The lethal yellow mutation (also abbreviated Ay) affects the agouti signalling protein which plays a major role in pigmentation. Heterozygous expression of it leads to the dominant expression of pheomelanin (which is yellow), causing the mice to express this color (among other effects). To understand why it is homozygous ...
Heterogeneity is a noun meaning:
the fact of consisting of parts or things that are very different from each other
In scientific use the context determines what is differing.
The context here is gene expression in individual embryonic stem cells (presumably single-cell RNASeq). Here the heterogeneity in expression would be for the genes
…(Neurod1, Klf4, ...
Let's first breakdown this question
What is CRISPR?
CRISPR is a group of DNA sequences that play a key role in the antiviral defense system of prokaryotic organisms such as bacteria and archaea.
They are derived from DNA fragments of bacteriophages that had previously infected the prokaryotes.
These sequences are used to destroy DNA from similar ...
What is Protein Expression Level?
This was the original title of the post, which I edited myself because I regard the answer as trivial, but the question as more substantial. To deal with the trivial first:
‘Level’ is not a scientific unit, and can only be used unambiguously
as a scientific term in its English sense in relation to liquids, e.g. “The level ...
What you are looking at there is a microarray dataset (see the description here).
Microarrays are chips that have many sequence specific probes on them and sometimes they have multiple probes for the same gene.
Readout differences between the probes of the same gene could be caused by either noise in the data or biological effects like expression differences ...