3
votes
How many photons does a GFP molecule absorb per second?
Since the quantum yield isn't the main focus here, I'll just take the Wikipedia figure of 0.79 photons emitted / photons absorbed
The rest can be worked out from first principles. The absorbance ...
- 2,485
2
votes
Expression of red and green fluorescences in the same tissue
These images are not typically taken using color cameras. They are taken using grayscale cameras, using filters that allow the excitation wavelengths to hit the sample and the emission wavelengths to ...
- 46.5k
1
vote
Accepted
Can GFP reporting be used to track localization of peptides in the ER, Golgi, and plasma membrane?
Historically speaking, the use of VSVG (Vesicular Stomatitis Virus G protein) labeled with GFP was indeed a practical method to define the secretory pathway. (@tyersome comment)
However, I am ...
- 606
1
vote
CRISPR/Cas9 edited E.coli on AFM?
Unless you're specifically targeting genes involved in the physical morphology of the cell, I doubt you would find any differences with CRISPRed vs non-CRISPRed cells that is measurable via AFM.
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- 177
1
vote
Fluorescent protein tags and colocalisation
"Are protein A and B co-located?" The answer is that it depends. Since your test question included the word "necessarily", this means that the answer to the test question is no. All you have to do is ...
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