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Difference between Peptone, Peptide and Proteose

Quick Answer Peptide refers to at least two amino acids joined by amide bonds. Proteoses are products of acid proteolysis which precipitate with saturating ammonium sulfate. Peptones are products of ...
canadianer's user avatar
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8 votes

Difference between Peptone, Peptide and Proteose

Let me first give the definitions of the three terms before differentiating them. Peptide: Peptides are biologically occurring short chains of amino acid monomers linked by peptide (amide) bonds1. ...
another 'Homo sapien''s user avatar
6 votes
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Is casein hydrolysate the same thing as casamino acids?

Roughly, yes they are similar, but there are processing differences- the real difference is filtering. A product containing simply casein hydrosylate will only be roughly filtered with lots of ...
Ben Welborn's user avatar
4 votes

How do absorbance readings indicate diauxic growth of bacteria?

How is growth rate an indicator of carbohydrate use? Carbohydrates are the carbon and energy source available for the microbes, and their assimilation is correlated to biomass production. The ...
Flo's user avatar
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4 votes

How pricey does the media need to be for a typical bacterial culture experiment?

It looks like Merck have different levels of quality control for their products. MQ 100 being the lowest up to MQ 600 being the highest. From what I can see the MQ200 product here has defined ...
JEJS's user avatar
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Medium preparation, using calcium hydroxide instead of potassium hydroxide

As @Chris said, $\ce{K+}$ salts are soluble in water whereas plenty of $\ce{Ca^{+2}}$ salts such as $\ce{CaSO4}$ and $\ce{Ca3(PO4)2}$ are insoluble in the aqueous medium. Therefore it leads to the ...
Amirreza Mousavi's user avatar
3 votes

How pricey does the media need to be for a typical bacterial culture experiment?

The answer depends on the application and level of oversight/compliance you require. Most research labs I've seen use de-ionized or even reverse-osmosis water (from a tap) for most culture media ...
MikeyC's user avatar
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3 votes
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Why isn't bacterial colony a monolayer?

As suggested by your conversation in the comments with anon, the bacterial colony is the outcome of a mechanistic growth model in which nutrient diffusion occurs from below to above. For an explicit ...
Maximilian Press's user avatar
3 votes

Autoclaving media Question

For the media bottle, it is better to keep the cap a bit loose to avoid to pressure inside the bottle. A good way is to had autoclaving tape on the side so that it is clear that the bottle has been ...
Dr. H. Lecter's user avatar
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Growth mediums required for human cell cultures

So this paper probably has what you need. Isolation and cultivation of human keratinocytes from skin or plucked hair for the generation of induced pluripotent stem cells The first part of that paper ...
Tom V.'s user avatar
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2 votes

Major effects on cells grown with 5% FBS supplemented media instead of 10% FBS?

If you were to run a flow-based viability assay (annexin V, live/dead fixable dyes, 7-AAD) or dual-fluorescence count (like acridine orange/propidium iodide, popular method as of late), you might find ...
CKM's user avatar
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How to create long term stock for Bacillus subtilis?

I worked with Bacillus subtilis WB800 and just used 50% glycerol / 50% LB. I imagine the same would work fine with wild-type. I would prepare the glycerol/LB mixture and autoclave it then create the ...
canadianer's user avatar
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1 vote

How pricey does the media need to be for a typical bacterial culture experiment?

This answer only concerns Streptococcus pneumoniae. The lab I worked in used cheap ingredients, the same as everyone else claimed to in this field. The only shocking thing I found was that the ...
Polypipe Wrangler's user avatar
1 vote

Would BMP7 introduced to a Petri dish with cartilage cause chondrocytes to replicate?

Hello and welcome Delaney Fitzpatrick. BMP7 - Bone morphogenetic protein 7 - Plays a role in cartilage homeostasis. Chondrocytes - A cell which has secreted the matrix of cartilage and become embedded ...
Andrew's user avatar
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1 vote

Growing Mouth Bacteria in a bowl of sugar water

I like the concept of this demonstration for teaching science! As rotaredom's comment pointed out, bacteria does not grow in plain water with sugar. Bacteria require a nitrogen source, trace minerals ...
Prashant Bharadwaj's user avatar
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A question about microbes growing media

You can put any media in a GasPak or other anaerobic system depending the type of anaerobic growth you are looking for. Sometimes blood is simply added to media to meet nutritional requirements of an ...
MikeyC's user avatar
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1 vote
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Is it safe to autoclave seawater agar?

I see no reason why it would precipitate chemically. Besides, you need to dissolve the agar via heat. Hence, auclaving is fine. If you do not believe me these protocol allow for autoclaving of ...
A. Radek Martinez's user avatar
1 vote

What culture medium should we use for tap/drinking water bacteria?

Usually when culture-based methods are used to test drinking water a range of selective culture media are used (media that favor the growth of a specific category of bacteria, e.g. the MacConkey Agar ...
Timothy Rozday's user avatar

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