6 votes
Accepted

Suggestion on program/algorithm to segment nuclei

The "Analyze Particles" function in FIJI might work. You will run into a few problems in any entirely automated analysis because your image isn't even brightness across the image; the ...
bob1's user avatar
  • 12k
4 votes
Accepted

COVID-19: size, real image

2019-nCoV also has enveloped virions that measure approximately 50–200 nm in diameter with a single positive-sense RNA genome. Epidemiological and clinical characteristics of 99 cases of 2019 novel ...
brazofuerte's user avatar
  • 1,582
3 votes

Detecting cancer in the hyperplasia phase using image processing and SEM

Not exactly spectroscopy, but microscopy is common for detecting pre-cancerous tissue as part of the standard Pap Test given to women at regular intervals throughout their life: https://en.wikipedia....
gringer's user avatar
  • 131
2 votes
Accepted

How are light sheet microscopy 3D images and their computed sections built up from stacks of 2D images?

According to their website dispim.org they are using registration software like the ImageJ Multiview-Reconstruction Plugin. Judging from the descriptions, this plugin uses the alignment of multiple ...
Frieke's user avatar
  • 1,127
1 vote
Accepted

Can you use images taken at different exposure times in western blot image analysis?

Generally no, you can't directly compare them and you can't easily compare across gels. I have done many hundreds of western blots, a large proportion of which were for quantitation, and in general ...
bob1's user avatar
  • 12k
1 vote
Accepted

What is the best way from remove background signal from a band when doing Western blot image analysis?

I haven't done immunoblots for years, and didn't do much quantitative analysis of them even then, so caveat emptor. However: 1) "Are there any ways to determine how wide you want the band for ...
Maximilian Press's user avatar
1 vote
Accepted

How does Line Integrate work on a laser scanning confocal microscope?

Most microscope software provides options for line average or line integrate as a function that helps the signal to noise ratio when taking an image. Averaging sums the lines and then divides by the ...
user1850479's user avatar
1 vote

How to measure the length of mitochondria from z stack fluorescent microscopy image?

ImageJ is usually the standard software to measure cell characteristics, a little bit of a learning curve but there is a large suite of analysis methods and image adjustments/filters.
Tom V.'s user avatar
  • 321

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