New answers tagged lab-techniques
6
According to the sigma document you referenced (emphasis added by me):
"The free acid is soluble at 0.24 g/L in water at 15ºC, while the sodium salt is soluble at >333 g/L in water at 15ºC. Therefore, pH control is very important to the use of this product. Aqueous solutions precipitate as the pH is lowered to 5."
According to the FDA document ...
0
I know you are asking for books, but for up-to-date basic overview of how some methods work - I would try websites or YouTube instead of textbooks...
One good website is BiteSizeBio. They have their own section for 'how to articles' and educational webinars. Otherwise I would also try YouTube. If you search in YouTube for basic techniques like 'how does the ...
2
This is done because at some point you start amplifying nonsense and not get a meaningful signal. If (and this is most often not the case) you have the ideal doubling of you DNA template in each cycle, you can calculate how little DNA you start with to detect it only around cycle 40 - or the other way round: What you will amplify and see as a "signal&...
2
If there is a way to:
Make the replication of a common culture organism (e.g. E. coli) dependent
upon the inhibitor or substrate of interest, possibly with a restrictive media.
Express the enzyme of interest in a relatively controlled manner in such organism (e.g. well caracterized promoter in plasmid with well characterized copy number),
Then you can get ...
0
A monograph from a pharmacopeia gives a good starting point for active pharmaceutical ingredient characterization. AFAIK there is no monograph for hCG nor your actual peptide of interest, but there is one for FSH (here for the european pharmacopeia monograph), which shares a subunit with CGA, meaning that modifications of it could suffice (though validating ...
2
Generally, sorbitol is one of those 'old tricks' in the book which is thrown into cultures or buffers for several reasons. Here are a few that I have heard of:
Bacterial cultures (e.g. E.coli BL21) that are optimised to express (produce) protein, because sorbitol is thought to increase the expression of soluble protein.
In buffers intended for proteins, as ...
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