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3 votes

Origin of the 260/280 ratio?

Here's one explanation: It's not the base composition that determines UV absorbance (260 nm), but whether the nucleic acid is in a single- or double stranded state. Promega cites Griffiths biochemical ...
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What are some experimental techniques to identify binding partners?

I will (1) obtain antibody of said PROTEIN (search catalog or custom-made). (2) pull down said PROTEIN with antibody and techniques you mentioned. (3) identify other proteins in the pulled-down ...
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