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2 votes
Accepted

Why does my cell subculture have these black dots like appearance?

I'm thinking you are very new to cell culture - your first step should be to ask people in your lab. You have a couple of problems here - it takes time for cells to settle and attach and start to ...
bob1's user avatar
  • 12.5k
2 votes
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In plasmid-based transient transfection of mesenchymal stem cells, do I have to select for transfected cells and verify GOI expression via a reporter?

No and Yes, respectively. No, you don't need to select for and generally you can't unless you use something like FACS to sort and capture cells expressing your marker/GOI. Transient transfections are ...
bob1's user avatar
  • 12.5k
1 vote
Accepted

Is it possible to cultivate cheek epithelial cells in a non-CO2 incubator?

L-15 media does not require a CO2 atmosphere and according to Sigma-Aldrich this media is suitable for primary explants of adult human tissues.
Sam's user avatar
  • 191

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