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3 votes

Mass Spectrometry (proteomics): How isotopes can be used to to determine charge of the peaks?

I've changed the order of your questions so that the answers follow from one another. What do the isotopes have to do with the charge? The presence of isotopes has no effect on the charge of the ...
Michael_A's user avatar
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3 votes
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Mass Spectrometry (proteomics): How isotopes can be used to to determine charge of the peaks?

If you have an ion of mass 100 and charge 2, the m/z ratio (let's exclude the root) will be 100/2=50. If you have isotopes, then you expect to find several peaks for a given fragment. For example, the ...
alec_djinn's user avatar
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2 votes
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MS/MS for complex samples

Liquid chromatography (LC) allows the peptides from a complex sample to be fed into the mass spectrometer over time. The bottom half of the image shows an MS spectrum from a single precursor scan and ...
Michael_A's user avatar
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1 vote
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Does mass spectrometry data have units?

I think MS data, like other spectroscopy and chromatography data, is usually quantified by comparing peak areas (in arbitrary units) to those of a quantitative standard. So the Y axis is just the ...
Ryan's user avatar
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1 vote

Are both B and Y ions required to determine the sequence of a protein by mass spectrometry?

In an MS/MS spectrum, difference between the successive peaks of a b or y ion series allows one to determine the amino acids making the series/peptide. Either one of them should be enough to give you ...
ktyagi's user avatar
  • 261

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