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10 votes

Is autoclaving sucrose solution necessary?

I wouldn't autoclave this solution - sucrose will partly break down into glucose and fructose. A portion of the sugars will also caramellize and color the solution yellow to brown. If you need the ...
Chris's user avatar
  • 51.7k
4 votes
Accepted

Why is EcoRI supplied with a unique buffer when it is allegedly 100% active in universal buffers?

Activity toward the desired substrate sequence is not the only concern in evaluating a restriction enzyme buffer system. In addition, you need to be concerned about so-called "star activity", or ...
R.M.'s user avatar
  • 1,554
4 votes

How do I calulcate the amount of concentrated stock solution to add to get the correct dilution?

Candianer's answer is a generally correct way of solving these problems but, in my opinion, overkill for this situation. All Nx buffers are mixed to make your life easier, and you will generally ...
Jack Aidley's user avatar
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4 votes
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How do I calulcate the amount of concentrated stock solution to add to get the correct dilution?

For any question regarding volume and concentration, you should consider the equation $C_1V_1=C_2V_2$. We can use it to derive a general equation for the addition of concentrated stock solutions. We ...
canadianer's user avatar
  • 17.8k
4 votes

Is there a reliable source for storage and stability of reducing agents like DTT?

Thiol-Based Reducing Agents The instability of thiol-based reducing agents in solution is due to their propensity to form disulfide bonds by the following half reaction: $$\ce{2RSH -> RSSR + 2H+ +...
canadianer's user avatar
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3 votes
Accepted

Opening coverslip package

Looking at the container from the side, it's probably a situation like this: There might be some clear plastic wrap around the entire thing as well that you would need to cut/break.
canadianer's user avatar
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3 votes
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Is autoclaving sucrose solution necessary?

I found a standard protocol for the same here : http://vosshall.rockefeller.edu/assets/file/Vosshall%20Lab%20Mosquito%20Rearing%20SOP%20DEC%2012-2014.pdf Take a look at page no.8. They suggest to ...
RKK's user avatar
  • 630
3 votes

Lab marker for labelling cell culture plastics?

I routinely use 70% ethanol and/or IPA to wipe off plastics written on with Fisherbrand fine-tip marking pens and I find they don't come off easily even after multiple sprays. As long as the marker ...
CKM's user avatar
  • 8,119
3 votes

Lab marker for labelling cell culture plastics?

This is hardcore going to come across as a SPAM answer, but I simply did a Google search: Laboratory Marking Pens from Ted Pella Statmark Pen™: resistant to formalin, ethanol isopropanol and xylene ...
theforestecologist's user avatar
3 votes

What kind of detergent should I use in cell culture laboratory for cleaning purposes?

I agree with Mike, but would like to add that if you are trying to do protein expression for purification, some detergents contain polyethylene glycol (PEG), which will readily bind to many proteins, ...
Ema's user avatar
  • 301
3 votes

What kind of detergent should I use in cell culture laboratory for cleaning purposes?

I can't tell if you're asking about glassware or work surfaces (hoods, benches etc), but... We use regular old dawn dish soap in my lab because what's more important than the soap you use to wash you'...
Mike's user avatar
  • 31
3 votes
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Ideal lab glassware cleaner for molecular biologist

If you're just using it for hand washing, you probably don't need an ultra-concentrated solution. A very small amount of detergent goes a long way. (Think about it - what would you do with an ultra-...
R.M.'s user avatar
  • 1,554
3 votes
Accepted

What do we call this adjustable platform used to ensure that something is positioned strictly level in a lab?

I remember using those for agarose gels as an undergraduate, though using them for PAGE doesn't make a lot of sense unless your bench is seriously out of level. Anyways, they can be called levelling ...
canadianer's user avatar
  • 17.8k
2 votes

Help with preparing Congo Red TSA

1) Yes, that's correct. 2) This is just a simple matter of applying the equation $C_1V_1=C_2V_2$. For congo red: $C_1=20\frac{mg}{mL}$ $C_2=0.04\frac{mg}{mL}$ $V_2=500\ mL$ I'm sure you and a ...
canadianer's user avatar
  • 17.8k
2 votes
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Cell dye in red fluorescence spectrum

I've used the ThermoFisher brand of CellTrace dyes. Specifically, I co-cultured two populations of cells, one with CellTrace Far Red in the APC channel and another with CellTrace Yellow in the PE ...
CKM's user avatar
  • 8,119
2 votes

Opening coverslip package

Basically the same answer, just explained differently... The top lifts off. There is a tiny lip around the bottom edge (all four sides opposite the label). It's just a matter of getting a grip well ...
Tonya's user avatar
  • 21
2 votes

Could a biological process organically build solid metal "organs"?

That is an excellent question. I was a little confused at first because, without realizing it, you actually cited a perfect example of a biological process that manipulates metal: bone formation. ...
CDB's user avatar
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1 vote

bunsen vs meker for best sterile field

The difference in flame diameter between a Bunsen and Meker (or Fisher) burner really isn't that much, so I'm not sure how much larger the sterile field would be. On the other hand, Fisher burners can ...
MattDMo's user avatar
  • 15.3k
1 vote

Cell dye in red fluorescence spectrum

There is a number of different dyes possible, as you can see in this figure (from here): A good overview can be found in the "Selecting Reagents for Multicolor Flow Cytometry" application note ...
Chris's user avatar
  • 51.7k

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