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5 votes

Why choose strawberries for DNA extraction?

There's more than one reason behind the use of strawberries: They are octoploid (8n), so they have eight copies of each chromosome per cell, i.e., there is a lot of DNA to isolate per unit of volume; ...
AliceD's user avatar
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4 votes
Accepted

Change of DNA concentration due to restriction digest?

Summary The poster wishes to know why spectrophotometric readings (presumably absorbance at 260 nm) on a sample of genomic DNA from an unspecified organism appear “slightly” greater after incubation ...
David's user avatar
  • 26.2k
3 votes

Change of DNA concentration due to restriction digest?

As you did a clean up, then you lost some DNA in this process. There are always losses from clean up steps. Note also that spectrophotometry is notoriously unreliable for DNA quantitation; it's more ...
bob1's user avatar
  • 12.4k
2 votes

Storing bacteria in a dry, room temperature long-term storage whilst maintaining viability

Lyophilization, aka freeze-drying, is probably the go-to method of microbial preservation without the need for cold storage. It's how bacterial strains purchased from commercial culture collections ...
MikeyC's user avatar
  • 4,744
2 votes

Method to detect Protein aggregation

I started writing a comment but it got out of hand so I'm just doing an answer. There are many many papers that describe methods for detecting aggregation. Below is a semi-random sample. What exactly ...
Maximilian Press's user avatar
1 vote

LCMS/MS versus Western Blot

Both LC/MS/MS and Western Blot methods can be applied to compare protein level changes in tissue samples across different treatment groups. Each method has pros and cons, as described below: Pros: • ...
Xiaoting Tang's user avatar
1 vote

Storing bacteria in a dry, room temperature long-term storage whilst maintaining viability

The answer to this strongly depends on the species of bacterium you are working with. The only ones that I know of that are capable of withstanding any dehydration process are those that form spores. ...
bob1's user avatar
  • 12.4k

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