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4

If the query genome is unknown, a microarray cannot be made for a target species. Microarrays have DNA fragments of what you want to amplify on them. Those fragments must be known. From nature: DNA microarrays are microscope slides that are printed with thousands of tiny spots in defined positions, with each spot containing a known DNA sequence or gene. ...


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It is entirely possible that different cell-lines express the same genes at drastically different levels. The proteinatlas provides data and analyses on differences between certain tissues or cell types / cell-lines. If your cells are of the same line/tissue, then they might still differ dependent on the cell-cycle, age and external factors. There are ...


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If I understand your question and graph correctly, your Y-axis is log(x/REF), where REF is some external standard. Your "Ref" on the x-axis you expect to be the same as REF, so that log(Ref/REF) "should be" zero, but you find it is not. However, it looks like the mean(log(Ref/REF)) is still approximately zero. This is what I would expect: ...


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