New answers tagged microarray
4
If the query genome is unknown, a microarray cannot be made for a target species. Microarrays have DNA fragments of what you want to amplify on them. Those fragments must be known.
From nature:
DNA microarrays are microscope slides that are printed with thousands
of tiny spots in defined positions, with each spot containing a known
DNA sequence or gene.
...
2
It is entirely possible that different cell-lines express the same genes at drastically different levels.
The proteinatlas provides data and analyses on differences between certain tissues or cell types / cell-lines.
If your cells are of the same line/tissue, then they might still differ dependent on the cell-cycle, age and external factors. There are ...
4
If I understand your question and graph correctly, your Y-axis is log(x/REF), where REF is some external standard. Your "Ref" on the x-axis you expect to be the same as REF, so that log(Ref/REF) "should be" zero, but you find it is not.
However, it looks like the mean(log(Ref/REF)) is still approximately zero. This is what I would expect: ...
Top 50 recent answers are included
