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An autoclave can sterilize both solids and liquids, whereas an oven with no pressure control is typically not suitable to sterilize liquids. Not only do you need to heat the chamber to 121°C, but you also need to make sure that the things you are trying to sterilize are not degraded by the treatment. For dry objects (e.g. glassware) you could heat the ...


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The bacterial cell wall is quite porous, and is not considered a permeability barrier for most small molecules. It mainly functions as structural support and to resist turgor pressures. The average pore size of relaxed peptidoglycans were measured to have a radius in a range of about 2.0 to 2.5 nm, regardless of thickness (i.e. Gram + and Gram - bacteria ...


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Answer No. There is ample evidence that: Most non-extremophilic bacteria grow over a broad range of external pH values, from 5.5 – 9.0, and maintain a cytoplasmic pH that lies within the narrow range of pH 7.4 – 7.8. The explanation for this is another matter, but, before discussing that question there are two points of possible misunderstanding in the ...


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it would be much simpler to further heat the steam at the ambient pressure of the autoclave room You cannot do that, physically. If you put water in a closed (water-vapor-tight) container, then heat it, the vapor "wants" to expand with growing temperature. But as it cannot expand your metal container (autoclave), the pressure goes up instead. It happens ...


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A good approach could be to use GC-MS to identify cytosolic metabolites. When focussing on cytosolic amino acids, it might be useful to precipitate the proteins in a purification step and continue with the soluble fraction. Subsequently, a derivatization is done. The mass spectrometry helps to better identify the amino acids. This article by Phelippe et al. ...


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As you say it is possible to increase the temperature of the steam over the saturation pressure. And with overheated steam, the instrument would reach the desired temperature as is the case in an autoclave. But the difference is how many time it would take to heat the sterilized instruments. And the difference is actually huge, we are talking of order of ...


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You could inoculate your sample in a selective medium or an enrichment medium to kill off the unwanted species. Of course, you will need to do a literature search to find the optimum medium for the species of your interest. Another option is to streak plates like so. With successive 'generations' of streaks, the inoculum gets diluted, so that you ultimately ...


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It's an interesting question. More interestingly there are a number of articles and a lot of studies are going on to prove that by manipulating oral microbiota, Caries or Periodontal diseases can be prevented. At this I want to point out as the OP has asked about the body's way of 'taming' the flora, I will share the references of the studies in which the ...


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