3 votes

Why do we choose to use agar instead of agarose in various microbial applications?

Agarose is indeed more expensive than agar because it is purified from agar, i.e. it is agar minus the agaropectin. Agaropectin is heterogeneous, and modified with acidic side-groups. The consequence ...
CaroZ's user avatar
  • 392
2 votes
Accepted

Does inoculum volume affect spread plate outcome?

Theoretically, for CFU determination the volume of the inoculum shouldn't matter as long as you plate a dilution series and find the plate with a countable number of colonies. It also shouldn't matter ...
MikeyC's user avatar
  • 4,714
1 vote

What's pooling in the spread plate technique?

Forming a pool (second definition in link); forming a small amount of liquid on a surface. A la "I knocked my cup over and the coffee formed a small pool on my bench" Basically this results ...
bob1's user avatar
  • 11.8k

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